Targeted Imaging of Estrogen Receptor-Positive Cancer Cells Using Fluorescent Estradiol Probes

IF 8 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Shabnam Mansuri, Subhadra Ojha, Sriram Kanvah
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引用次数: 0

Abstract

Breast cancer remains the second most common cause of cancer-related deaths in women worldwide, with ≈70% of cases linked to the overexpression of Estrogen Receptor (ERα). Existing imaging tools often fail to reliably differentiate between ER-positive and ER-negative cancer cells. To address this limitation, two novel fluorescent probes, E2N and E2R, are synthesized by conjugating estradiol to styryl and rhodamine-based fluorophores using click chemistry. These probes are characterized by their photophysical properties, biocompatibility, and selective targeting of ER-positive cells. Cellular uptake studies demonstrate preferential internalization of E2N and E2R in ER-positive MCF-7, ZR-75-1, and T-47D cells, with minimal uptake in ER-negative MDA-MB-231, MDA-MB-468, and healthy COS-7 and NIH-3T3 cell lines. Kinetic studies reveal efficient and rapid uptake of E2N in ER-positive MCF-7 cells, while mechanistic investigations identified clathrin-mediated endocytosis as the receptor-mediated pathway for both probes. Localization studies further confirm their mitochondrial specificity in ER-positive cells, with E2R displaying higher mitochondrial selectivity. These findings underscore the potential of E2N and E2R as powerful tools for distinguishing ER-positive from ER-negative breast cancer cells. Their receptor-mediated targeting and precise imaging capabilities make them promising candidates for advancing breast cancer diagnostics and enabling more targeted therapeutic strategies.

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来源期刊
Advanced Optical Materials
Advanced Optical Materials MATERIALS SCIENCE, MULTIDISCIPLINARY-OPTICS
CiteScore
13.70
自引率
6.70%
发文量
883
审稿时长
1.5 months
期刊介绍: Advanced Optical Materials, part of the esteemed Advanced portfolio, is a unique materials science journal concentrating on all facets of light-matter interactions. For over a decade, it has been the preferred optical materials journal for significant discoveries in photonics, plasmonics, metamaterials, and more. The Advanced portfolio from Wiley is a collection of globally respected, high-impact journals that disseminate the best science from established and emerging researchers, aiding them in fulfilling their mission and amplifying the reach of their scientific discoveries.
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