Real-Time Photocatalytic Measurement of Dental Materials in an Open System.

M-Y Lee, H-W Yoon, H Cai, S-J Shin, J-S Kwon
{"title":"Real-Time Photocatalytic Measurement of Dental Materials in an Open System.","authors":"M-Y Lee, H-W Yoon, H Cai, S-J Shin, J-S Kwon","doi":"10.1177/00220345251319320","DOIUrl":null,"url":null,"abstract":"<p><p>It is common to encounter discrepancies between in vitro and in vivo studies, particularly when assessing the antibiofilm efficacy of dental materials. Typically, dental materials are tested in a closed system where fresh nutrients are not replenished, the test conditions are static, and the same planktonic bacteria persist. However, real environments are characterized by the continuous supply of fresh nutrients, dynamic saliva flow, and the periodic removal of planktonic bacteria through swallowing. To address these differences, we used an open system approach using microfluidic chips that simulate the nutrient and fluid flow conditions of the mouth. This setup enables the spatiotemporal development of biofilms, facilitates real-time observation, and provides deeper insights into the biofilm formation and removal processes. Photocatalytic dental materials are particularly suitable for use with microfluidic chips, as these devices allow real-time tracking of biofilm dynamics, both with and without light exposure. Nitrogen-doped titanium dioxide effectively produces reactive oxygen species (ROS) under visible light conditions, even when embedded in a resin matrix. These ROS have been shown to inhibit <i>Enterococcus faecalis</i> biofilms. The evaluation of the photocatalytic effects of dental materials using microfluidic chips showed that both new and established biofilms were disrupted by ROS production. ROS weakens the interface between the biofilm and dental material, allowing the biofilm mass to be removed by fluid flow. Furthermore, the open system provided by microfluidic chips demonstrated higher accuracy in evaluating antibiofilm efficiency than the conventional system did. Thus, the developed microfluidic chip is a novel and promising tool for assessing antibiofilm properties, with potential applications in various fields.</p>","PeriodicalId":94075,"journal":{"name":"Journal of dental research","volume":" ","pages":"220345251319320"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of dental research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/00220345251319320","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

It is common to encounter discrepancies between in vitro and in vivo studies, particularly when assessing the antibiofilm efficacy of dental materials. Typically, dental materials are tested in a closed system where fresh nutrients are not replenished, the test conditions are static, and the same planktonic bacteria persist. However, real environments are characterized by the continuous supply of fresh nutrients, dynamic saliva flow, and the periodic removal of planktonic bacteria through swallowing. To address these differences, we used an open system approach using microfluidic chips that simulate the nutrient and fluid flow conditions of the mouth. This setup enables the spatiotemporal development of biofilms, facilitates real-time observation, and provides deeper insights into the biofilm formation and removal processes. Photocatalytic dental materials are particularly suitable for use with microfluidic chips, as these devices allow real-time tracking of biofilm dynamics, both with and without light exposure. Nitrogen-doped titanium dioxide effectively produces reactive oxygen species (ROS) under visible light conditions, even when embedded in a resin matrix. These ROS have been shown to inhibit Enterococcus faecalis biofilms. The evaluation of the photocatalytic effects of dental materials using microfluidic chips showed that both new and established biofilms were disrupted by ROS production. ROS weakens the interface between the biofilm and dental material, allowing the biofilm mass to be removed by fluid flow. Furthermore, the open system provided by microfluidic chips demonstrated higher accuracy in evaluating antibiofilm efficiency than the conventional system did. Thus, the developed microfluidic chip is a novel and promising tool for assessing antibiofilm properties, with potential applications in various fields.

开放系统中牙科材料的实时光催化测量。
在体外和体内研究中经常遇到差异,特别是在评估牙科材料的抗生物膜功效时。通常,牙科材料是在一个封闭的系统中进行测试的,在这个系统中,新鲜的营养物质没有得到补充,测试条件是静态的,同样的浮游细菌持续存在。然而,真实环境的特点是新鲜营养物质的持续供应,唾液的动态流动,以及浮游细菌通过吞咽的周期性清除。为了解决这些差异,我们使用了一种开放系统方法,使用微流控芯片来模拟口腔的营养和流体流动条件。该装置实现了生物膜的时空发展,便于实时观察,并对生物膜的形成和去除过程提供了更深入的了解。光催化牙科材料特别适合与微流控芯片一起使用,因为这些设备允许实时跟踪生物膜动力学,无论是否有光照射。氮掺杂的二氧化钛在可见光条件下有效地产生活性氧(ROS),即使嵌入在树脂基体中。这些活性氧已被证明能抑制粪肠球菌生物膜。利用微流控芯片对牙科材料的光催化效果进行评价,发现新的和已建立的生物膜都被ROS的产生所破坏。活性氧削弱了生物膜和牙科材料之间的界面,使生物膜团被流体除去。此外,微流控芯片提供的开放系统在评估抗生素膜效率方面比传统系统具有更高的准确性。因此,所开发的微流控芯片是一种新型的、有前途的抗生素膜性能评估工具,在各个领域都有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信