Cargo-less Nanoparticles Prevent Bone Loss in Periodontitis and Peri-implantitis.

M H A Saleh, A Roh, K Martin, M Mianecki, A Tariq Sheikh, P Singh, K Akorede, M N Saunders, L D Shea, A Decker, J T Decker
{"title":"Cargo-less Nanoparticles Prevent Bone Loss in Periodontitis and Peri-implantitis.","authors":"M H A Saleh, A Roh, K Martin, M Mianecki, A Tariq Sheikh, P Singh, K Akorede, M N Saunders, L D Shea, A Decker, J T Decker","doi":"10.1177/00220345251319256","DOIUrl":null,"url":null,"abstract":"<p><p>Periodontal and peri-implant diseases are a significant public health problem worldwide, resulting in the destruction of the supporting bone. These bone defects can cause esthetic problems, increased relapse rate, and eventually tooth loss. The etiology of periodontal disease involves an influx of innate immune cells (neutrophils and monocytes) and upregulation of local inflammatory cytokines in the gingiva. Biodegradable polymeric nanoparticles are an inexpensive, safe, and effective means of preventing innate immune activation by bacterial biofilms. We therefore hypothesize that this technology is a potential means of managing periodontal disease. Polylactic acid (PLA) particles were fabricated using an oil-in-water emulsion and used as a therapy in ligature-induced periodontitis and peri-implantitis. Mice were treated daily with nanoparticles or saline control through intravenous injection for 5 or 7 d. Bone loss and quality were characterized using micro-computed tomography and histology, and immune cell infiltrate was characterized by flow cytometry and enzyme-linked immunosorbent assay. PLA particle therapy prevented bone loss in both periodontitis and peri-implantitis. Particle treatment was associated with decreased osteoclast activation. Flow cytometry showed particles were mainly taken up by macrophages and limited inflammatory monocyte recruitment to the ligature site. In vitro evaluation of particle therapy demonstrated the inhibition of toll-like receptor activation during particle treatment. These results extended to monocytes that had been presensitized by titania nanoparticles. Taken together, the results of these experiments demonstrated that cargo-less PLA particle therapy may be a safe, cost-effective therapy to manage inflammatory bone loss in periodontal disease.</p>","PeriodicalId":94075,"journal":{"name":"Journal of dental research","volume":" ","pages":"220345251319256"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of dental research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1177/00220345251319256","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Periodontal and peri-implant diseases are a significant public health problem worldwide, resulting in the destruction of the supporting bone. These bone defects can cause esthetic problems, increased relapse rate, and eventually tooth loss. The etiology of periodontal disease involves an influx of innate immune cells (neutrophils and monocytes) and upregulation of local inflammatory cytokines in the gingiva. Biodegradable polymeric nanoparticles are an inexpensive, safe, and effective means of preventing innate immune activation by bacterial biofilms. We therefore hypothesize that this technology is a potential means of managing periodontal disease. Polylactic acid (PLA) particles were fabricated using an oil-in-water emulsion and used as a therapy in ligature-induced periodontitis and peri-implantitis. Mice were treated daily with nanoparticles or saline control through intravenous injection for 5 or 7 d. Bone loss and quality were characterized using micro-computed tomography and histology, and immune cell infiltrate was characterized by flow cytometry and enzyme-linked immunosorbent assay. PLA particle therapy prevented bone loss in both periodontitis and peri-implantitis. Particle treatment was associated with decreased osteoclast activation. Flow cytometry showed particles were mainly taken up by macrophages and limited inflammatory monocyte recruitment to the ligature site. In vitro evaluation of particle therapy demonstrated the inhibition of toll-like receptor activation during particle treatment. These results extended to monocytes that had been presensitized by titania nanoparticles. Taken together, the results of these experiments demonstrated that cargo-less PLA particle therapy may be a safe, cost-effective therapy to manage inflammatory bone loss in periodontal disease.

无货物纳米颗粒预防牙周炎和种植周炎的骨质流失。
牙周和种植体周围疾病是世界范围内的一个重大公共卫生问题,导致支撑骨的破坏。这些骨缺损会引起审美问题,增加复发率,最终导致牙齿脱落。牙周病的病因涉及先天免疫细胞(中性粒细胞和单核细胞)的涌入和牙龈局部炎症细胞因子的上调。可生物降解聚合物纳米颗粒是一种廉价、安全、有效的防止细菌生物膜激活先天免疫的方法。因此,我们假设这项技术是管理牙周病的潜在手段。采用水包油乳液制备聚乳酸(PLA)颗粒,用于结扎性牙周炎和种植周炎的治疗。小鼠每天静脉注射纳米颗粒或生理盐水,持续5或7天。通过显微计算机断层扫描和组织学研究骨质流失和质量,通过流式细胞术和酶联免疫吸附试验研究免疫细胞浸润。聚乳酸颗粒治疗可预防牙周炎和种植周炎的骨质流失。颗粒治疗与破骨细胞活性降低有关。流式细胞术显示颗粒主要被巨噬细胞吸收,炎性单核细胞向结扎部位募集有限。粒子治疗的体外评价表明,在粒子治疗期间,toll样受体的激活受到抑制。这些结果延伸到被二氧化钛纳米颗粒呈现的单核细胞。综上所述,这些实验的结果表明,无货聚乳酸颗粒治疗可能是一种安全、经济有效的治疗牙周病炎症性骨质流失的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信