Investigation of TLR4 Polymorphism in Children with Vesicoureteral Reflux and Renal Scarring.

IF 0.5 4区 医学 Q4 GENETICS & HEREDITY
Balkan Journal of Medical Genetics Pub Date : 2025-03-06 eCollection Date: 2024-12-01 DOI:10.2478/bjmg-2024-0013
N M Sav, R Eroz, Duran N Kalay, O Kilicaslan, Karaca S Erisen
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引用次数: 0

Abstract

Vesicoureteral reflux (VUR) is an important factor in the etiology of recurrent urinary tract infections (UTIs). Permanent kidney damage may develop in children with high-grade VUR in the long term. This damage may progress with the development of scar tissue in some patients. The TLR4 gene is an important resistance mechanism, especially against UTIs. TLR4 gene polymorphism is associated with recurrent UTIs and kidney scar development in the long term. This study aimed to examine the relationship between scar development and TLR4 gene polymorphism in children with VUR. This cross-sectional study included 49 patients with recurrent UTIs and primary vesicoureteral reflux. Patients were divided into two groups (26 patients with the scar, and 23 patients without scar) according to the presence of scar tissue. TLR4 gene polymorphisms of the patients were evaluated by Next Generation Sequencing. The TLR4 gene polymorphism was significantly higher in the compound heterozygous group with scarring than in the group without scarring (p=0.03). Gene polymorphisms, c.958T>C, c.942A>G, c.776A>G, c.1076C>T, c.896AT, c.1078C>T were presented more commonly in the group with scarring. Moreover, gene polymorphisms c.942A>G and c.776A>G were defined for the first time in this study among patients with scar tissue. The higher incidence of some TLR4 gene polymorphisms in patients with scarring suggested that these variations might cause permanent kidney damage. In addition to genetic predisposition, environmental factors such as untreated UTIs might also contribute to scar formation.

膀胱输尿管反流和肾瘢痕形成患儿TLR4多态性的研究
膀胱输尿管反流(VUR)是复发性尿路感染(uti)的重要病因。长期来看,高级别VUR患儿可能出现永久性肾损害。在一些患者中,这种损伤可能随着瘢痕组织的发展而进展。TLR4基因是一种重要的耐药机制,尤其是针对uti。TLR4基因多态性与尿路感染复发和肾瘢痕的长期发展有关。本研究旨在探讨VUR患儿瘢痕发育与TLR4基因多态性的关系。这项横断面研究包括49例复发性尿路感染和原发性膀胱输尿管反流患者。根据有无瘢痕组织分为两组,有瘢痕组26例,无瘢痕组23例。采用Next Generation Sequencing检测患者TLR4基因多态性。有瘢痕形成的复合杂合组TLR4基因多态性显著高于无瘢痕形成组(p=0.03)。C . 958t >C、C . 942a >G、C . 776a >G、C . 1076c >T、C . 896at、C . 1078c >T基因多态性在瘢痕组中更为常见。此外,本研究首次在瘢痕组织患者中定义了c.942A>G和c.776A>G基因多态性。一些TLR4基因多态性在疤痕患者中的较高发生率表明,这些变异可能导致永久性肾损伤。除了遗传易感性,环境因素,如未经治疗的尿路感染也可能导致疤痕的形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.00
自引率
0.00%
发文量
0
审稿时长
>12 weeks
期刊介绍: Balkan Journal of Medical Genetics is a journal in the English language for publication of articles involving all branches of medical genetics: human cytogenetics, molecular genetics, clinical genetics, immunogenetics, oncogenetics, pharmacogenetics, population genetics, genetic screening and diagnosis of monogenic and polygenic diseases, prenatal and preimplantation genetic diagnosis, genetic counselling, advances in treatment and prevention.
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