Nonhematopoietic Stem Cell Identification and Sorting in Adult Retina.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4386-0_5

Ana Flores, Laura Fernández-Sánchez, Oksana Kutsyr, Alberto Yáñez, María Luisa Gil, Daniel Gozalbo, Victoria Maneu, Pedro Lax

引用次数: 0

Abstract

Flow cytometry enables the detection and characterization of specific cell populations, and proper identification of particular cell types can be assessed by immunohistochemistry. Here, we describe the identification and sorting of nonhematopoietic stem cells expressing stem cell antigen-1 from adult murine retinas using flow cytometry and immunohistochemistry strategies. These sorted cells can be further used for regenerative purposes.

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成人视网膜非造血干细胞的鉴定与分选。

流式细胞术能够检测和表征特定的细胞群，并且可以通过免疫组织化学评估特定细胞类型的正确鉴定。在这里，我们描述了使用流式细胞术和免疫组织化学方法从成年小鼠视网膜中鉴定和分选表达干细胞抗原-1的非造血干细胞。这些经过分类的细胞可以进一步用于再生目的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.