Development of multiparametric bioprinting method for generation of 3D printed cell-laden structures.

IF 2.5 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sophie Lipshutz, Yoontae Kim, Micaila Curtis, Leanne Friedrich, Stella Alimperti
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引用次数: 0

Abstract

The organ transplantation field requires new approaches for replacing and regenerating tissues due to the lack of adequate transplant methods. Three-dimensional (3D) extrusion-based bioprinting is a rapid prototyping approach that can engineer 3D  scaffolds for tissue regeneration applications. In this process, 3D printed cell-based constructs, consisting of biomaterials, growth factors, and cells, are formed by the extrusion of bioinks from nozzles. However, extrusion applies shear stresses to cells, often leading to cellular damage or membrane rupture. To address this limitation, herein, we developed and optimized a 3D bioprinting approach by evaluating the effect of key extrusion-based 3D bioprinting parameters-bioink viscosity, nozzle size, shape, and printing speed-on cell viability. Our results revealed  that cells printed in higher-viscosity bioinks, with smaller, cylindrical nozzles, exhibited lower viability due to their exposure to high shear stresses. Translational flow speed had a cell-dependent impact, as different cell types have different sensitivities to the magnitude and duration of shear stress inside the nozzle. Overall, evaluating these parameters could facilitate the development of 3D high-resolution bioprinted constructs for tissue regeneration applications, offering a more efficient alternative to traditional fabrication methods, which are often labor intensive, expensive, and repetitive.

3D打印细胞负载结构的多参数生物打印方法的发展。
由于缺乏足够的移植方法,器官移植领域需要新的方法来替代和再生组织。三维(3D)基于挤压的生物打印是一种快速原型方法,可以设计用于组织再生应用的3D支架。在这个过程中,3D打印的基于细胞的结构,包括生物材料、生长因子和细胞,通过从喷嘴中挤出生物墨水形成。然而,挤压对细胞施加剪切应力,经常导致细胞损伤或膜破裂。为了解决这一限制,我们开发并优化了一种3D生物打印方法,通过评估基于挤压的3D生物打印参数(生物墨水粘度、喷嘴尺寸、形状和打印速度)对细胞活力的影响。我们的研究结果表明,在高粘度生物墨水中打印的细胞,使用较小的圆柱形喷嘴,由于暴露在高剪切应力下,表现出较低的活力。由于不同的细胞类型对喷嘴内剪切应力的大小和持续时间的敏感性不同,平移流速具有细胞依赖性的影响。总的来说,评估这些参数可以促进用于组织再生应用的3D高分辨率生物打印结构的开发,为传统的制造方法提供更有效的替代方法,这些方法通常是劳动密集型的,昂贵的,重复的。
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来源期刊
Biotechnology Progress
Biotechnology Progress 工程技术-生物工程与应用微生物
CiteScore
6.50
自引率
3.40%
发文量
83
审稿时长
4 months
期刊介绍: Biotechnology Progress , an official, bimonthly publication of the American Institute of Chemical Engineers and its technological community, the Society for Biological Engineering, features peer-reviewed research articles, reviews, and descriptions of emerging techniques for the development and design of new processes, products, and devices for the biotechnology, biopharmaceutical and bioprocess industries. Widespread interest includes application of biological and engineering principles in fields such as applied cellular physiology and metabolic engineering, biocatalysis and bioreactor design, bioseparations and downstream processing, cell culture and tissue engineering, biosensors and process control, bioinformatics and systems biology, biomaterials and artificial organs, stem cell biology and genetics, and plant biology and food science. Manuscripts concerning the design of related processes, products, or devices are also encouraged. Four types of manuscripts are printed in the Journal: Research Papers, Topical or Review Papers, Letters to the Editor, and R & D Notes.
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