Immunological biomarkers at birth and later risk of celiac disease.

IF 2.5 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY
Maria Ulnes, Veroniqa Lundbäck, Susanne Lindgren, Mattias Molin, Rolf H Zetterström, Olov Ekwall, Karl Mårild
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引用次数: 0

Abstract

Background: The role of immune cell profiles at birth in determining the risk of celiac disease (CD) development is currently unestablished. This study aimed to determine the associations between T- and B-cell profiles at birth and pediatric CD.

Methods: This regional cohort study analyzed prospectively collected dried blood spots from 158 children with CD (median 7 years old at CD diagnosis) and two matched comparators each (n = 316). We quantified T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles (KRECs) as measures of thymic and bone marrow output at birth. Moreover, we used epigenetic cell counting to estimate the percentages of lymphocyte subsets: CD3+, CD4+, CD8 + T cells, CD4 + memory T, regulatory T, B, and NK cells.

Results: No associations were found between measured immune cell markers at birth and CD development (all p values > 0.26). The median number of copies was 120 for TRECs (IQR = 92-168) and 136 (IQR = 91-183) for CD patients and comparators, respectively, and for KRECs, it was 69 (IQR = 45-100) for CD patients and 66 for comparators (IQR = 44-93). Across the groups, there were similar median percentages of T cells (CD, 32.6% [IQR = 27.0-43.8%] vs. comparators, 33.9% [IQR = 26.3-45.7%]) and B cells (CD, 25.4% [IQR = 20.3-30.6%] vs. comparators, 24.7% [IQR = 19.9-30.8%]). The ratio of the lymphocyte subset estimates between CD patients and comparators approximated one; all p values were > 0.26. The results were consistent across strata defined by sex, HLA type, and age at diagnosis.

Conclusion: Genetic and epigenetic markers for B cells and T cells in immune cell profiles at birth did not impact susceptibility to childhood-onset CD.

出生时的免疫生物标志物和后来乳糜泻的风险。
背景:出生时免疫细胞谱在确定乳糜泻(CD)发展风险中的作用目前尚未确定。该研究旨在确定出生时T细胞和b细胞谱与儿童CD之间的关系。方法:该区域队列研究前瞻性地分析了158名CD患儿(诊断时中位年龄为7岁)和两名匹配的比较者(n = 316)的干血斑。我们量化了t细胞受体切除圈(trec)和kappa删除重组切除圈(KRECs)作为出生时胸腺和骨髓输出量的测量。此外,我们使用表观遗传细胞计数来估计淋巴细胞亚群的百分比:CD3+、CD4+、CD8 + T细胞、CD4+记忆T细胞、调节性T细胞、B细胞和NK细胞。结果:出生时测量的免疫细胞标记物与乳糜病的发展没有关联(p值均为> 0.26)。CD患者和比较组的TRECs中位拷贝数分别为120份(IQR = 92-168)和136份(IQR = 91-183),而KRECs中位拷贝数CD患者为69份(IQR = 45-100),比较组为66份(IQR = 44-93)。各组间T细胞和B细胞的中位数百分比相似(CD为32.6% [IQR = 27.0-43.8%],对照组为33.9% [IQR = 26.3-45.7%]), CD为25.4% [IQR = 20.3-30.6%],对照组为24.7% [IQR = 19.9-30.8%])。在CD患者和比较者之间的淋巴细胞亚群估计值的比率接近1;p值均为0.26。结果是一致的跨阶层定义的性别,HLA类型和年龄诊断。结论:出生时免疫细胞谱中B细胞和T细胞的遗传和表观遗传标记并不影响儿童期发病CD的易感性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Gastroenterology
BMC Gastroenterology 医学-胃肠肝病学
CiteScore
4.20
自引率
0.00%
发文量
465
审稿时长
6 months
期刊介绍: BMC Gastroenterology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of gastrointestinal and hepatobiliary disorders, as well as related molecular genetics, pathophysiology, and epidemiology.
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