Warming stimulates cellulose decomposition by recruiting phylogenetically diverse but functionally similar microorganisms.

Abstract

Cellulose is the most abundant component of plant litter, which is critical for terrestrial carbon cycling. Nonetheless, it remains unknown how global warming affects cellulose-decomposing microorganisms. Here, we carried out a 3-year litterbag experiment to examine cellulose decomposition undergoing +3°C warming in a tallgrass prairie. Most cellulose-associated bacteria and fungi in litterbags were also detected in bulk soil, and bacteria in litterbags had higher community-level rrn copy numbers, larger genome sizes, and higher genome guanine-cytosine (GC) contents than those in bulk soil, implying higher growth rates. Warming stimulated soil respiration by 32.3% and accelerated mass loss of cellulose, concurring with the increase in relative abundances of most functional genes associated with carbon decomposition in litterbags. Incorporating cellulose-decomposing genes into an ecosystem model reduced model parameter uncertainty and showed that warming stimulated microbial biomass, activity, and soil carbon decomposition. Collectively, our study supports a trait-centric view since cellulose-decomposing genes or genomic traits are amenable for ecosystem modeling. By characterizing the phylogenetically diverse yet functionally similar cellulose-associated microorganisms and their responses to warming, we take a step toward more precise predictions of soil carbon dynamics under future climate scenarios.