You Lu , Li Qi , QinZheng Xu , ZhuoHuan Li , Hao Duan , Fei He , Na Zhao , James M. Hyman
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引用次数: 0
Abstract
The enzyme-linked immunosorbent assay (ELISA) is a cornerstone technique for quantifying protein secretion in biological research. However, the built-in software provided by ELISA plate readers often struggles to accurately detect low-concentration proteins, particularly in the sub-nanogram/mL range, due to limitations in calibration curve fitting. We developed the ELISA Protein Detector (EPD) to overcome these challenges. This open-source Python-based software employs advanced optimization algorithms to enhance curve fitting precision, particularly at low detection thresholds.” EPD features an intuitive user interface, requires minimal technical expertise, and supports robust cross-validation to enhance the reliability of ELISA data analysis. Tested on Windows systems, this tool provides a cost-effective and versatile solution for researchers, enabling accurate quantification of low-level protein concentrations and addressing the shortcomings of standard ELISA software in diverse biological and clinical applications.
期刊介绍:
The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens. (2) Purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies. (5) Localizing antigens and/or antibodies in tissues and cells. (6) Detecting, and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Documenting cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. (12) Molecular techniques for studying immune cells and their receptors. (13) Imaging of the immune system. (14) Methods for production or their fragments in eukaryotic and prokaryotic cells.
In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note. Review articles will also be published by the Journal of Immunological Methods. In general these manuscripts are by solicitation however anyone interested in submitting a review can contact the Reviews Editor and provide an outline of the proposed review.