Identification of active ingredients from Lamiophlomis rotata that increase wound repair in mouse skin

IF 6.8 2区医学 Q1 PHARMACOLOGY & PHARMACY

British Journal of Pharmacology Pub Date : 2025-03-10 DOI:10.1111/bph.17452

Huan Li, Chang Chen, Xinzhu Chen, Jiale Liu, Chen Luo, Huilin Chen, Xiaoyu Geng, Zheng Pan

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引用次数: 0

Abstract

Background and Purpose

We previously showed that the iridoid glycoside extract of Lamiophlomis rotata (IGLR) induces M2 macrophage polarisation to accelerate wound healing. The potentially active compounds (quality markers) in IGLR that accelerate wound healing have not been elucidated; we here identified quality markers that accelerate wound healing.

Experimental Approach

After IGLR gavage of dorsal excisional wound mice and normal mice for 7 days, the tissues were analysed using the matrix-assisted laser desorption/ionisation (MALDI) mass spectrometry imaging (MSI) method and were further validated by liquid chromatography/mass spectrometry (LC/MS) coupled with multivariate analyses to identify quality markers.

Key Results

Using the MSI assay, Shanzhiside methyl ester (SM), 8-O-acetylshanzhiside methyl ester (ASM) and Phlorigidoside C (PhC) were identified as the quality markers in IGLR and overlapped with endogenous metabolites (lactate, citrate and itaconate) in the same class by UMAP manifold analysis. Additionally, the results were also confirmed by UPLC-Q/TOF-MS coupled with multivariate analyses of the skin tissues in normal and wound groups. MSI data from the livers and kidneys revealed that the accumulation of SM, ASM and PhC in the livers significantly increased in the model group, and the ion intensity of their glucuronide conjugates in the kidneys was decreased compared to the normal group. These results suggested that bioavailability of these compounds was improved in the wound group.

Conclusions and Implications

The overall data demonstrated that SM, ASM and PhC were selectively increased in new granulation tissues, and are most likely the quality markers that accelerate wound healing.

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促进小鼠皮肤创面修复活性成分的鉴定。

背景和目的：我们之前发现，环烯醚萜苷提取物的Lamiophlomis rotata （IGLR）诱导M2巨噬细胞极化，加速伤口愈合。IGLR中加速伤口愈合的潜在活性化合物（质量标记）尚未阐明；我们在这里发现了加速伤口愈合的质量标记。实验方法：IGLR灌胃背部切除伤口小鼠和正常小鼠7天后，采用基质辅助激光解吸/电离（MALDI）质谱成像（MSI）方法对组织进行分析，并进一步采用液相色谱/质谱（LC/MS）结合多变量分析进行验证，以确定质量标记物。关键结果：MSI法鉴定山芝苷甲酯（SM）、8- o -乙酰山芝苷甲酯（ASM）和根草苷C （PhC）为IGLR的质量标记，UMAP流形分析鉴定其与内源代谢物（乳酸、柠檬酸和衣壳酸）重叠。此外，UPLC-Q/TOF-MS结合正常组和创面组皮肤组织的多变量分析也证实了这一结果。肝脏和肾脏的MSI数据显示，与正常组相比，模型组肝脏中SM、ASM和PhC的蓄积明显增加，其葡萄糖醛酸偶联物在肾脏中的离子强度降低。这些结果表明，这些化合物的生物利用度在伤口组得到改善。结论和意义：总体数据表明，SM、ASM和PhC在新生肉芽组织中选择性增加，很可能是加速伤口愈合的质量标志。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

British Journal of Pharmacology 医学-药学

CiteScore

15.40

自引率

12.30%

发文量

270

审稿时长

2.0 months

期刊介绍： The British Journal of Pharmacology (BJP) is a biomedical science journal offering comprehensive international coverage of experimental and translational pharmacology. It publishes original research, authoritative reviews, mini reviews, systematic reviews, meta-analyses, databases, letters to the Editor, and commentaries. Review articles, databases, systematic reviews, and meta-analyses are typically commissioned, but unsolicited contributions are also considered, either as standalone papers or part of themed issues. In addition to basic science research, BJP features translational pharmacology research, including proof-of-concept and early mechanistic studies in humans. While it generally does not publish first-in-man phase I studies or phase IIb, III, or IV studies, exceptions may be made under certain circumstances, particularly if results are combined with preclinical studies.