{"title":"[Acupuncture regulates dynamic flux of Ca<sup>2+</sup>, Na<sup>+</sup>, and H<sub>2</sub>O<sub>2</sub> in skeletal muscle injury induced by eccentric exercise in rats].","authors":"Xue-Lin Zhang, Qian Zhao, Ai-Shan Liu, Ming-Liang Duan, Jing-Jing Ding, Hua Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate the effects of acupuncture on dynamic changes in Ca<sup>2+</sup>, Na<sup>+</sup>, and H<sub>2</sub>O<sub>2</sub> flux following eccentric exercise-induced muscle injury. The total of 324 healthy male Wistar rats were randomly divided into 6 groups: control group (C), eccentric exercise group (E), eccentric exercise with acupuncture group (EA), EA with TRP channel blocker group (EAT), EA with NOX2 blocker group (EAN) and EA with placebo group (EAP). Gastrocnemius muscles were subject to lengthening contractions with percutaneous electrical stimulation, followed by immediate pretreatment with blocking agents. After 30 min, acupuncture needling was administered to the gastrocnemius muscle, and real-time dynamic changes of Ca<sup>2+</sup>, Na<sup>+</sup> and H<sub>2</sub>O<sub>2</sub> flux were measured with non-invasive micro-test technique during the needle retention period, immediately, 3 h, 6 h, and 24 h post-extraction respectively. Results showed that compared with the E group, acupuncture significantly increased net Ca<sup>2+</sup> efflux (<i>P</i> < 0.05), extended the period of net Na<sup>+</sup> influx, and significantly decreased net H<sub>2</sub>O<sub>2</sub> efflux (<i>P</i> < 0.05). However, these effects were significantly attenuated in the EAT and EAN groups, where excessive net H<sub>2</sub>O<sub>2</sub> efflux was observed (<i>P</i> < 0.001). These findings indicate that acupuncture regulates the dynamic changes of Ca<sup>2+</sup>, Na<sup>+</sup> and H<sub>2</sub>O<sub>2</sub> flux by activating the TRP channels and interacting with NOX2 activity following eccentric exercise-induced skeletal muscle injury.</p>","PeriodicalId":7134,"journal":{"name":"生理学报","volume":"77 1","pages":"47-61"},"PeriodicalIF":0.0000,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"生理学报","FirstCategoryId":"1087","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to investigate the effects of acupuncture on dynamic changes in Ca2+, Na+, and H2O2 flux following eccentric exercise-induced muscle injury. The total of 324 healthy male Wistar rats were randomly divided into 6 groups: control group (C), eccentric exercise group (E), eccentric exercise with acupuncture group (EA), EA with TRP channel blocker group (EAT), EA with NOX2 blocker group (EAN) and EA with placebo group (EAP). Gastrocnemius muscles were subject to lengthening contractions with percutaneous electrical stimulation, followed by immediate pretreatment with blocking agents. After 30 min, acupuncture needling was administered to the gastrocnemius muscle, and real-time dynamic changes of Ca2+, Na+ and H2O2 flux were measured with non-invasive micro-test technique during the needle retention period, immediately, 3 h, 6 h, and 24 h post-extraction respectively. Results showed that compared with the E group, acupuncture significantly increased net Ca2+ efflux (P < 0.05), extended the period of net Na+ influx, and significantly decreased net H2O2 efflux (P < 0.05). However, these effects were significantly attenuated in the EAT and EAN groups, where excessive net H2O2 efflux was observed (P < 0.001). These findings indicate that acupuncture regulates the dynamic changes of Ca2+, Na+ and H2O2 flux by activating the TRP channels and interacting with NOX2 activity following eccentric exercise-induced skeletal muscle injury.
期刊介绍:
Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.