Engineering Chromatin Regulation of Xylose Utilization in Budding Yeast Saccharomyces cerevisiae for Efficient Bioconversion.

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2025-03-10 DOI:10.1021/acssynbio.4c00730

Wei-Bin Wang, Rui-Qi Tang, Bing Yuan, Yue Wang, Guo-Dong Liu, Dong-Min Li, Hong-Jia Zhang, Xin-Qing Zhao, Feng-Wu Bai

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引用次数: 0

Abstract

Utilization of xylose as a renewable carbon source has received constant interest. Considering that the structure and state of eukaryotic chromatin are inextricably intertwined, it is significant to explore chromatin regulation for engineering xylose metabolism in yeast. Here, we show that two chromatin remodelers, namely, Swr1 and Isw1, affect xylose utilization in recombinant budding yeastSaccharomyces cerevisiae. Overexpressing SWR1 showed the highest increase in xylose utilization, up to 29.3%, compared to that of the parent strain. Furthermore, comparative transcriptome and chromatin immunoprecipitation sequencing (ChIP-seq) analyses revealed significantly different changes of gene expression by elevated expression of Swr1 and Isw1. Reduced histone H2A.Z occupancy in two key carbon-metabolism regulators of Mig2 and Sip2 was further observed in the engineered yeast. Further tests showed improved xylose utilization of the engineered yeast in the presence of corncob hydrolysate. Our results suggest that chromatin regulators are critical genetic elements in recombinant S. cerevisiae for engineering xylose metabolism.

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在芽殖酵母中进行木糖利用的染色质调控工程，以实现高效的生物转化。

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.