Biological evaluation of a new highly sensitive and selective fluorescent probe for hypochlorous acid and its imaging application in cell and zebrafish

IF 4.5 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioorganic Chemistry Pub Date : 2025-03-08 DOI:10.1016/j.bioorg.2025.108358

Min Zheng , Huayan Wen , Zhengxiang Meng , Wenli Guo , Kun Wang , Miaohui Yu , Ke Li , Yun Zhang , Kechun Liu , Lei Cai , Baocun Zhu , Wenlong Sheng

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引用次数: 0

Abstract

Hypochlorous acid is one of the most widely distributed reactive oxygen species in vivo. It is usually used as a signal molecule to participate in various life activities such as immunity and metabolism, and plays a notable role in maintaining homeostasis. When hypochlorous acid level is abnormal in the body, it will lead to a variety of diseases, such as Parkinson's disease, Alzheimer's disease, atherosclerosis and cancer. Therefore, it is necessary to develop a bio-friendly fluorescent probe with fast sensitivity and specific accuracy. In this study, the innovative probe PRS owns good optical properties, sensitivity and selectivity, and the response mechanism that the generation of new bond enhanced the fluorescence intensity is studied. Biocompatibility of probe is systematically and innovatively evaluated by using cells and zebrafish models. Note that the biocompatibility valuation of probe results from cytotoxicity test, zebrafish behavioral test, hepatotoxicity test, cardiotoxicity test, nephrotoxicity test, blood vessel toxicity test, immunotoxicity test and neurotoxicity test, and experimental indicators like swimming duration, swimming distance, swimming speed, pericardial rub, fractional shortening, stroke volume, heart rate, SV-BA, shortening rate of the ventricular short axis, liver area, liver fluorescence intensity, total length of intersegmental vessels, number of vessels, and average vessel length show that the probe has good biocompatibility. Moreover, the detection performance of the probe shows that the probe can target hypochlorous acid in cell and zebrafish models. The probe is proved to be much essential for the monitoring of hypochlorous acid in vivo. Therefore, it has been proven that the meaningful detection of probe PRS for HOCl is promising in the living organism. Moreover, our innovative biocompatibility testing can be used to evaluate the biosafety of fluorescent probe as well.

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.