Autocrine Wingless constricts the Drosophila embryonic gut by Ca+2-mediated repolarisation of mesoderm cells.

IF 6.5 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
EMBO Reports Pub Date : 2025-04-01 Epub Date: 2025-03-07 DOI:10.1038/s44319-025-00411-x
Delia Ricolo, Francesca Tamba, Jordi Casanova
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引用次数: 0

Abstract

Wg/Wnt signalling-a highly conserved transduction pathway-has most commonly been found to be involved in patterning, cell fate, or cell proliferation, but less so in shaping organs or body parts. A remarkable case of the latter is the role of Wg signalling in the midgut of the Drosophila embryo. The Drosophila embryonic midgut is divided into four chambers that arise by the formation of three constrictions at distinct sites along the midgut. In particular, Wg is responsible for the middle constriction, a role first described more than 30 years ago. However, while some partial data have been obtained regarding the formation of this gut constriction, an overall picture of the process is lacking. Here we unveil that Wg signalling leads to this constriction by inducing ClC-a transcription in a subset of mesodermal cells. ClC-a, encodes a chloride channel, which in turn prompts a Ca+2 pulse in these cells. Consequently, the mesoderm cells, which already showed some polarity, repolarise and in so doing so they reshape the microtubule organisation, therefore inducing the constriction of the cells.

自分泌无翅通过Ca+2介导的中胚层细胞重极化收缩果蝇胚胎肠。
Wg/Wnt信号是一种高度保守的转导途径,通常被发现与模式、细胞命运或细胞增殖有关,但在器官或身体部位的形成中较少。后者的一个显著的例子是Wg信号在果蝇胚胎中肠中的作用。果蝇胚胎中肠分为四个腔室,这些腔室由沿中肠不同部位形成的三个收缩而产生。特别是,Wg负责中间收缩,这是30多年前首次描述的角色。然而,虽然已经获得了一些关于这种肠道收缩形成的部分数据,但缺乏对该过程的全面了解。在这里,我们揭示了Wg信号通过在中胚层细胞亚群中诱导ClC-a转录而导致这种收缩。ClC-a编码一个氯离子通道,该通道反过来在这些细胞中激发Ca+2脉冲。因此,已经显示出极性的中胚层细胞重新极化,在这样做的过程中,它们重塑了微管组织,从而诱导细胞收缩。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
EMBO Reports
EMBO Reports 生物-生化与分子生物学
CiteScore
11.20
自引率
1.30%
发文量
267
审稿时长
1 months
期刊介绍: EMBO Reports is a scientific journal that specializes in publishing research articles in the fields of molecular biology, cell biology, and developmental biology. The journal is known for its commitment to publishing high-quality, impactful research that provides novel physiological and functional insights. These insights are expected to be supported by robust evidence, with independent lines of inquiry validating the findings. The journal's scope includes both long and short-format papers, catering to different types of research contributions. It values studies that: Communicate major findings: Articles that report significant discoveries or advancements in the understanding of biological processes at the molecular, cellular, and developmental levels. Confirm important findings: Research that validates or supports existing knowledge in the field, reinforcing the reliability of previous studies. Refute prominent claims: Studies that challenge or disprove widely accepted ideas or hypotheses in the biosciences, contributing to the correction and evolution of scientific understanding. Present null data: Papers that report negative results or findings that do not support a particular hypothesis, which are crucial for the scientific process as they help to refine or redirect research efforts. EMBO Reports is dedicated to maintaining high standards of scientific rigor and integrity, ensuring that the research it publishes contributes meaningfully to the advancement of knowledge in the life sciences. By covering a broad spectrum of topics and encouraging the publication of both positive and negative results, the journal plays a vital role in promoting a comprehensive and balanced view of scientific inquiry. 
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