Acetic acid production from corn straw via enzymatic degradation using putative acetyl esterase from the metagenome assembled genome

IF 3.4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Enzyme and Microbial Technology Pub Date : 2025-03-04 DOI:10.1016/j.enzmictec.2025.110619

Hao Chen , Zhiwei Zeng , Rup Lal , Jie Wu , Jia Chen , Mei Li , Lulu Cao , Xiqiang Liu , Ruzhe Zhang , Chunjie Gong

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Abstract

Acetic acid production from corn straw by enzyme catalysis shows its application value in food industry. In this study, a gene encoding for a putative acetyl esterase derived from Sphingobacterium soilsilvae Em02 was discovered in metagenome assembled genome. The gene was expressed in Escherichia coli BL21 to obtain enzyme with a molecular mass of 38.8 kDa. P-Nitrophenyl acetate was used as a substrate to determine the enzyme activity. The enzyme demonstrated optimal activity under conditions of 40 °C and a neutral pH of 7.0. Under optimal conditions, 17.58 mg of acetic acid was obtained using the enzyme from 50 mg corn straw pretreated with amylase. The acetyl esterase derived from Sphingobacterium soilsilvae Em02, demonstrates significant potential for biotechnological applications, particularly in biomass degradation.

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利用酶催化从玉米秸秆中生产乙酸显示了其在食品工业中的应用价值。本研究在元基因组中发现了一个编码推测乙酰酯酶的基因，该基因来源于Sphingobacterium soilsilvae Em02。将该基因在大肠杆菌 BL21 中表达，获得了分子质量为 38.8 kDa 的酶。以对硝基苯乙酸酯为底物测定酶的活性。该酶在 40 °C、中性 pH 值为 7.0 的条件下表现出最佳活性。在最佳条件下，从 50 毫克用淀粉酶预处理的玉米秸秆中使用该酶可获得 17.58 毫克乙酸。从 Sphingobacterium soilsilvae Em02 中提取的乙酰酯酶具有很大的生物技术应用潜力，特别是在生物质降解方面。

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来源期刊

Enzyme and Microbial Technology 生物-生物工程与应用微生物

CiteScore

7.60

自引率

5.90%

发文量

142

审稿时长

38 days

期刊介绍： Enzyme and Microbial Technology is an international, peer-reviewed journal publishing original research and reviews, of biotechnological significance and novelty, on basic and applied aspects of the science and technology of processes involving the use of enzymes, micro-organisms, animal cells and plant cells. We especially encourage submissions on: Biocatalysis and the use of Directed Evolution in Synthetic Biology and Biotechnology Biotechnological Production of New Bioactive Molecules, Biomaterials, Biopharmaceuticals, and Biofuels New Imaging Techniques and Biosensors, especially as applicable to Healthcare and Systems Biology New Biotechnological Approaches in Genomics, Proteomics and Metabolomics Metabolic Engineering, Biomolecular Engineering and Nanobiotechnology Manuscripts which report isolation, purification, immobilization or utilization of organisms or enzymes which are already well-described in the literature are not suitable for publication in EMT, unless their primary purpose is to report significant new findings or approaches which are of broad biotechnological importance. Similarly, manuscripts which report optimization studies on well-established processes are inappropriate. EMT does not accept papers dealing with mathematical modeling unless they report significant, new experimental data.