Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep.

IF 3.7 2区生物学 Q2 MICROBIOLOGY

Microbiology spectrum Pub Date : 2025-04-01 Epub Date: 2025-03-07 DOI:10.1128/spectrum.03268-24

Jing Wu, Xiaoming Zhang, Khan Ayesha, Shahzad Khuram, Jianzhao Cui, Gaofu Wang, Zhaxi Yangzong, Mingyan Shi, Xunping Jiang, Long Li, Guiqiong Liu, Wangsheng Zhao, Tianzeng Song

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Abstract

Rumen flatulence is a diet-related rumen disease in ruminants. This study induced a rumen flatulence model in Xizang sheep using alfalfa (HRF) and wheatgrass (MRF). The aim was to understand the rumen microbiota diversity in healthy and pathological states, host-microbiota interactions, and the molecular mechanisms of rumen flatulence. Results showed that the pH in the HRF and MRF groups was lower than that in the natural grass group (LRF). SCFA concentrations varied between groups: in HRF, 2-BA and CA increased; in MRF, 4-MVA and 5-MCA rose. Microbial analysis indicated that the alpha- and beta-diversity of HRF and MRF groups were lower than LRF's, with different microbial compositions. Transcriptome analysis revealed many differentially expressed genes (DEGs). Compared to MRF, HRF had 348 upregulated and 511 downregulated DEGs. Versus LRF, MRF had 201 upregulated and 185 downregulated DEGs, while HRF had 128 upregulated and 238 downregulated DEGs. Spearman's correlation analysis showed that there was a positive correlation between Butyrivibrio, Quinella, and specific genes. These findings reveal the potential mechanism of rumen flatulence in Xizang sheep and provide new insights into the prevention and treatment of the disease.IMPORTANCEThe research used a high-protein diet to induce a model to understand the diversity of rumen microbiota and its interaction with the host, as well as exploring the molecular mechanisms of rumen flatulence.

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苜蓿和小麦诱导西藏羊瘤胃胀气机理的多组学分析。

瘤胃胀气是反刍动物的一种与饲料有关的瘤胃疾病。本研究采用苜蓿（HRF）和小麦草（MRF）分别诱导西藏羊瘤胃胀气模型。目的是了解健康和病理状态下瘤胃微生物群的多样性、宿主-微生物群的相互作用以及瘤胃胀气的分子机制。结果表明，HRF组和MRF组的pH值低于天然草组（LRF）。各组间SCFA浓度存在差异：HRF组2-BA、CA升高；MRF中4-MVA、5-MCA升高。微生物分析表明，HRF组和MRF组的α和β多样性低于LRF组，且微生物组成不同。转录组分析显示许多差异表达基因（DEGs）。与MRF相比，HRF有348个上调deg， 511个下调deg。与LRF相比，MRF有201个上调和185个下调的deg，而HRF有128个上调和238个下调的deg。Spearman相关分析表明，丁酸弧菌、奎尼拉菌与特定基因之间存在正相关。这些发现揭示了西藏羊瘤胃胀气的潜在机制，为该疾病的预防和治疗提供了新的见解。本研究通过高蛋白饲粮诱导模型，了解瘤胃微生物群的多样性及其与宿主的相互作用，探索瘤胃胀气的分子机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbiology spectrum Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.20

自引率

5.40%

发文量

1800

期刊介绍： Microbiology Spectrum publishes commissioned review articles on topics in microbiology representing ten content areas: Archaea; Food Microbiology; Bacterial Genetics, Cell Biology, and Physiology; Clinical Microbiology; Environmental Microbiology and Ecology; Eukaryotic Microbes; Genomics, Computational, and Synthetic Microbiology; Immunology; Pathogenesis; and Virology. Reviews are interrelated, with each review linking to other related content. A large board of Microbiology Spectrum editors aids in the development of topics for potential reviews and in the identification of an editor, or editors, who shepherd each collection.