No toxic effects or interactions between aflatoxin B1 and zearalenone in broiler chickens fed diets at China's regulatory limits

IF 3 4区医学 Q1 MEDICINE, LEGAL

Regulatory Toxicology and Pharmacology Pub Date : 2025-03-03 DOI:10.1016/j.yrtph.2025.105799

Wanjun Zhang, Yongpeng Jin, Ying Liu, Ruiqi Tan, Gaoyi Liu, Wenjun He, Sunlin Luo, Yutong Tang, Qiao Chen, Yiqiang Chen

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引用次数: 0

Abstract

Currently, several countries such as China have established regulatory limits for six major mycotoxins in animal feed. However, these limits are primarily designed for single mycotoxin exposure. Co-contamination with multiple mycotoxins is increasingly common, and simultaneous exposure may lead to additive toxic effects. This study aimed to investigate the effects of individual and combined contamination of aflatoxin B₁ (AFB₁) and zearalenone (ZEA) at China's regulatory limits on the growth performance, oxidative stress, serum biochemistry, immunity indicators, intestinal morphology, liver and kidney histopathology of broilers by incorporating mycotoxin standards into the feed. A total of 432 one-day-old male AA broilers were randomly assigned to four treatment groups, each consisting of six replicates of 18 birds. The control group received a basic diet, while the experimental groups were supplemented with 10 μg/kg AFB₁, 500 μg/kg ZEA, or a combination of both in the basic diet. The experimental period lasted for 35 days. The results revealed no significant differences among the groups in terms of growth performance, oxidative damage markers, serum biochemistry, cytokine levels, intestinal health, or histopathological assessments of the liver and kidneys. Furthermore, no toxic interactions between the two mycotoxins were observed. Taken together, these results suggest that future assessments should take into account the combined toxicological effects of a wider range of mycotoxins to inform the revision and formulation of feed safety standards.

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来源期刊

Regulatory Toxicology and Pharmacology 医学-毒理学

CiteScore

6.70

自引率

8.80%

发文量

147

审稿时长

58 days

期刊介绍： Regulatory Toxicology and Pharmacology publishes peer reviewed articles that involve the generation, evaluation, and interpretation of experimental animal and human data that are of direct importance and relevance for regulatory authorities with respect to toxicological and pharmacological regulations in society. All peer-reviewed articles that are published should be devoted to improve the protection of human health and environment. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of toxicological and pharmacological compounds on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of human and environmental health. Types of peer-reviewed articles published: -Original research articles of relevance for regulatory aspects covering aspects including, but not limited to: 1.Factors influencing human sensitivity 2.Exposure science related to risk assessment 3.Alternative toxicological test methods 4.Frameworks for evaluation and integration of data in regulatory evaluations 5.Harmonization across regulatory agencies 6.Read-across methods and evaluations -Contemporary Reviews on policy related Research issues -Letters to the Editor -Guest Editorials (by Invitation)