Relationship Between Apoptosis Induction in Cancer Cells and the Amount of Electrical Stimulation on Frequency Analysis of Nanosecond Pulsed Electric Field

IF 0.4 4区工程技术 Q4 ENGINEERING, ELECTRICAL & ELECTRONIC

Electrical Engineering in Japan Pub Date : 2025-01-06 DOI:10.1002/eej.23493

Yuto Ninagawa, Ren Sugiura, Eiko Kato, Ippei Yagi, Kohki Nagata, Hidehiko Yamaoka, Kosuke Tachibana, Akinori Oda, Satoshi Uchida

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引用次数: 0

Abstract

Nanosecond pulse electric field (nsPEF) therapy has attracted attention in recent years as a noninvasive cancer treatment with minimal side effects. However, the relationship between the electrical stress induced in cells by the external electric field and the biological response has not been fully investigated quantitatively. We investigated the effects of nsPEF therapy on cancer cells using microgap electrodes under low-voltage conditions. We developed an equivalent circuit model using intracellular components to determine the amount of stimulation of cellular components and performed frequency analysis of nsPEF. nsPEF applied to Jurkat cells activated caspase-3 and induced apoptosis at shorter pulse widths. nsPEF induced biological responses and apoptosis in cancer cells. The relationship between the biological response induced by nsPEF and the amount of electrical stimulation was investigated by comparing the experimental results with frequency analysis. Using quantitative values for each cellular element, the relationship between the biological response and the amount of electrical stimulation can be integrated.

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基于纳秒脉冲电场频率分析的肿瘤细胞凋亡诱导与电刺激量的关系

纳秒脉冲电场（nsPEF）作为一种副作用小、无创的癌症治疗方法近年来备受关注。然而，外界电场在细胞内诱导的电应激与生物反应之间的关系尚未得到充分的定量研究。我们研究了在低电压条件下使用微间隙电极的nsPEF治疗对癌细胞的影响。我们利用细胞内成分建立了等效电路模型，以确定细胞成分的刺激量，并对nsPEF进行了频率分析。应用于Jurkat细胞的nsPEF在较短的脉冲宽度下激活caspase-3并诱导细胞凋亡。nsPEF诱导肿瘤细胞的生物学反应和凋亡。通过对比实验结果和频率分析，探讨了nsPEF诱发的生物反应与电刺激量的关系。利用每个细胞元素的定量值，生物反应和电刺激量之间的关系可以整合起来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Electrical Engineering in Japan 工程技术-工程：电子与电气

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Electrical Engineering in Japan (EEJ) is an official journal of the Institute of Electrical Engineers of Japan (IEEJ). This authoritative journal is a translation of the Transactions of the Institute of Electrical Engineers of Japan. It publishes 16 issues a year on original research findings in Electrical Engineering with special focus on the science, technology and applications of electric power, such as power generation, transmission and conversion, electric railways (including magnetic levitation devices), motors, switching, power economics.