Chemical profile and bioactivity enhancement of Eucalyptus camaldulensis essential oils through hybridization and rectification: A chemometric and molecular docking study

IF 3.5 2区农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Food and Bioproducts Processing Pub Date : 2025-02-27 DOI:10.1016/j.fbp.2025.02.012

Fatima Ez-Zahra Aabassi, Mouhcine Fadil, Ayoub El-Mrabet, Taoufiq Saffaj, Mohamed Amine Mechatte, Abdellah Farah

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引用次数: 0

Abstract

The rectification of essential oils (EOs) through fractional distillation emerges as a promising approach for enhancing their compositional quality and biological efficacy. This physicochemical separation process enables the selective concentration of desirable bioactive compounds while simultaneously reducing the presence of unwanted constituents, thereby yielding fractions with optimized chemical profiles. This study investigated the semi-industrial rectification effects on EOs from Eucalyptus camaldulensis leaves and its hybrid Clone 583 through fractional distillation. Chemical analysis revealed higher yield in the hybrid (0.75 %) compared to the parent species (0.46 %), with 1.8-cineole and α-pinene identified as principal compounds among 38 detected constituents. Rectification yielded higher 1.8-cineole concentrations in hybrid fractions (85 % vs. 59 %) while reducing α-pinene content (5.10 % vs. 19.20 %). Clone 583 exhibited superior antibacterial activity against M. smegmatis (F_c: 76.36 ± 0.08 mm vs. F₆: 68.36 ± 0.17 mm) and enhanced antioxidant properties in DPPH assays (IC₅₀: F_c: 25.21 ± 0.05 µg/ml vs. F₆: 44.00 ± 0.08 µg/ml). Principal Component Analysis and Hierarchical Cluster Analysis indicated 1.8-cineole as the primary contributor to antioxidant activity, while molecular docking suggested that both 1.8-cineole and α-pinene exert antibacterial effects through LasR-OC12 binding. These findings demonstrate that the combination of hybridization and rectification techniques present significant potential for various industrial applications, particularly in pharmaceutical, cosmetic, and food preservation sectors, where high-purity bioactive compounds are essential.

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来源期刊

Food and Bioproducts Processing 工程技术-工程：化工

CiteScore

9.70

自引率

4.30%

发文量

115

审稿时长

24 days

期刊介绍： Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.