Co-exposure to a honeybee pathogen and an insecticide: synergistic effects in a new solitary bee host but not in Apis mellifera.

IF 3.8 1区 生物学 Q1 BIOLOGY
Rafaela Tadei, Giovanni Cilia, Elaine Cristina Mathias da Silva, Gonzalo Sancho Blanco, Sergio Albacete, Celeste Azpiazu, Anna Granato, Francesca Bortolin, Antonio Martini, Jordi Bosch, Osmar Malaspina, Fabio Sgolastra
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Abstract

Pesticides and pathogens are major drivers of bee declines. However, their potential interactions are poorly understood, especially for non-Apis bees. This study assessed the combined effects of infestation by the honeybee pathogen Vairimorpha ceranae and chronic exposure to the insecticide flupyradifurone on Osmia bicornis and Apis mellifera. We investigated whether V. ceranae could reproduce in a new solitary bee host (O. bicornis) and assessed sublethal and lethal effects of the pathogen and the pesticide, alone and in combination. We also analysed the interactive effects of the combined exposure on V. ceranae proliferation and bee survival in the two bee species. Newly emerged bees were orally infected with 100 000 spores of V. ceranae and then exposed ad libitum to flupyradifurone at field-realistic concentrations. We showed, for the first time to our knowledge, that V. ceranae can replicate in the midgut of O. bicornis, causing histological damage, impaired phototactic response, reduced food consumption and decreased longevity. The pathogen-pesticide combination caused a synergistic effect in O. bicornis, leading to an abrupt survival decline. In A. mellifera, V. ceranae and flupyradifurone showed antagonistic survival effects, but the pesticide promoted pathogen proliferation. Our results warn against the potential effects of pathogen spillover and multiple stressor exposure on non-Apis bees.

共同暴露于一种蜜蜂病原体和一种杀虫剂:对一个新的独居蜜蜂寄主的协同效应,但在蜜蜂中没有。
杀虫剂和病原体是蜜蜂数量减少的主要原因。然而,人们对它们之间的潜在相互作用知之甚少,尤其是对非api蜜蜂。本研究评估了蜜蜂病原体蜜蜂变异螨(Vairimorpha ceranae)侵染和长期暴露于杀虫剂氟吡喃醌对双角蚁(Osmia bicornis)和蜜蜂(Apis mellifera)的综合影响。研究了蜜蜂弧菌能否在新的独居蜜蜂寄主(双角蜜蜂)中繁殖,并评估了该病原体与农药单独和联合使用的亚致死效应和致死效应。我们还分析了联合暴露对两种蜂群中蜜蜂弧菌增殖和蜜蜂存活的交互影响。新出现的蜜蜂经口感染了100,000个ceranae孢子,然后随意暴露于实际浓度的氟吡地黄酮中。据我们所知,这是第一次表明,ceranae可以在双角o.s bicornis的中肠中复制,导致组织损伤、光致反应受损、食物消耗减少和寿命缩短。病原菌与农药的组合对双角夜蛾产生了协同效应,导致其存活率急剧下降。在蜜蜂中,蜜蜂弧菌和氟吡喃醌表现出拮抗生存作用,但农药促进了病原体的增殖。我们的研究结果警告了病原体溢出和多重应激源暴露对非api蜜蜂的潜在影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.90
自引率
4.30%
发文量
502
审稿时长
1 months
期刊介绍: Proceedings B is the Royal Society’s flagship biological research journal, accepting original articles and reviews of outstanding scientific importance and broad general interest. The main criteria for acceptance are that a study is novel, and has general significance to biologists. Articles published cover a wide range of areas within the biological sciences, many have relevance to organisms and the environments in which they live. The scope includes, but is not limited to, ecology, evolution, behavior, health and disease epidemiology, neuroscience and cognition, behavioral genetics, development, biomechanics, paleontology, comparative biology, molecular ecology and evolution, and global change biology.
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