CeDaD-a novel assay for simultaneous tracking of cell death and division in a single population.

IF 6.1 2区生物学 Q1 CELL BIOLOGY

Cell Death Discovery Pub Date : 2025-03-04 DOI:10.1038/s41420-025-02370-7

Lukas Nöltner, Kurt Engeland, Robin Kohler

引用次数: 0

Abstract

The cell division cycle and the various forms of programmed cell death are interconnected. A prominent example is the tumor suppressor p53, which not only induces apoptosis but also plays an important role in the arrest of the cell cycle. Consequently, simultaneous analysis of cell division and cell death is frequently of significant interest in cell biology research. Traditionally, these processes require distinct assays, making concurrent analysis challenging. To address this, we present a novel combined assay, called CeDaD assay-Cell Death and Division assay-which allows for the simultaneous quantification of cell division and cell death within a single-cell population. This assay utilizes a straightforward flow cytometric approach, combining a staining based on carboxyfluorescein succinimidyl ester (CFSE) to monitor cell division with an annexin V-derived staining to assess the extent of cell death.

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cedad是一种同时跟踪单个群体中细胞死亡和分裂的新方法。

细胞分裂周期和各种形式的程序性细胞死亡是相互联系的。一个突出的例子是肿瘤抑制因子p53，它不仅诱导细胞凋亡，而且在细胞周期阻滞中起着重要作用。因此，细胞分裂和细胞死亡的同时分析经常是细胞生物学研究的重要兴趣。传统上，这些过程需要不同的分析，使得并发分析具有挑战性。为了解决这个问题，我们提出了一种新的联合检测方法，称为CeDaD检测——细胞死亡和分裂检测——它可以同时定量单细胞群体中的细胞分裂和细胞死亡。该试验采用简单的流式细胞术方法，结合基于羧基荧光素琥珀酰酰酯（CFSE）的染色来监测细胞分裂，并结合膜联蛋白v衍生的染色来评估细胞死亡的程度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Death Discovery Biochemistry, Genetics and Molecular Biology-Cell Biology

CiteScore

8.30

自引率

1.40%

发文量

468

审稿时长

9 weeks

期刊介绍： Cell Death Discovery is a multidisciplinary, international, online-only, open access journal, dedicated to publishing research at the intersection of medicine with biochemistry, pharmacology, immunology, cell biology and cell death, provided it is scientifically sound. The unrestricted access to research findings in Cell Death Discovery will foster a dynamic and highly productive dialogue between basic scientists and clinicians, as well as researchers in industry with a focus on cancer, neurobiology and inflammation research. As an official journal of the Cell Death Differentiation Association (ADMC), Cell Death Discovery will build upon the success of Cell Death & Differentiation and Cell Death & Disease in publishing important peer-reviewed original research, timely reviews and editorial commentary. Cell Death Discovery is committed to increasing the reproducibility of research. To this end, in conjunction with its sister journals Cell Death & Differentiation and Cell Death & Disease, Cell Death Discovery provides a unique forum for scientists as well as clinicians and members of the pharmaceutical and biotechnical industry. It is committed to the rapid publication of high quality original papers that relate to these subjects, together with topical, usually solicited, reviews, editorial correspondence and occasional commentaries on controversial and scientifically informative issues.