Preβ1-high-density lipoprotein binds to TG-rich lipoproteins and its release is impaired in the postprandial state among patients with poorly controlled type 2 diabetes.

IF 2.1 4区医学 Q3 MEDICAL LABORATORY TECHNOLOGY

Annals of Clinical Biochemistry Pub Date : 2025-03-04 DOI:10.1177/00045632251328154

Yuna Horiuchi, Satoshi Hirayama, Atsushi Hori, Yuri Ichikawa, Satoshi Soda, Utako Seino, Kazumasa Sekihara, Tsuyoshi Ueno, Yoshifumi Fukushima, Katsuo Kubono, Takashi Miida

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Abstract

BackgroundAlthough preβ1-high-density lipoprotein (preβ1-HDL) promotes cholesterol efflux, high fasting preβ1-high-density lipoprotein levels after breakfast are reduced in patients with poorly controlled type 2 diabetes.ObjectiveThis study investigated whether preβ1-high-density lipoprotein binds to triglyceride (TG)-rich lipoproteins (TGRLs) in the postprandial state and is released during lipolysis.MethodsWe measured preβ1-high-density lipoprotein concentrations, lecithin-cholesterol acyltransferase (LCAT) activity, and LCAT-dependent preβ1-high-density lipoprotein conversion before and after breakfast in patients with diabetes. We also performed in vitro studies using TGRLs. Preβ1-high-density lipoprotein was quantified by enzyme-linked immunosorbent assay and native two-dimensional gradient gel (N-2D-gel) electrophoresis.ResultsBefore breakfast, the diabetes group had higher preβ1-high-density lipoprotein concentrations than the healthy controls; after breakfast, levels in the two groups were similar. Neither LCAT mass nor the LCAT-dependent preβ1-high-density lipoprotein conversion rate changed after breakfast. Mixing of fasting plasma with chylomicrons or very-low-density lipoprotein (VLDL) reduced the preβ1-high-density lipoprotein level by 15% ± 4% and 45% ± 10%, respectively. N-2D-gel electrophoresis showed that preβ1-high-density lipoprotein was generated by bacteria-derived TG lipase only from postprandial VLDL of patients with type 2 diabetes.ConclusionPreβ1-high-density lipoprotein binds to TGRLs in the postprandial state and is released during lipolysis, implying that postprandial hyperlipidemia impairs reverse cholesterol transport in patients with poorly controlled type 2 diabetes.

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在控制不良的2型糖尿病患者中，前β1- hdl与富含tg的脂蛋白结合，其释放在餐后状态受损。

背景：虽然β1-高密度脂蛋白（pre - β1- hdl）促进胆固醇外排，但控制不良的2型糖尿病患者早餐后空腹β1- hdl水平较高。目的：本研究探讨pre - β1- hdl是否在餐后状态下与富含甘油三酯（TG）的脂蛋白（tgrl）结合，并在脂解过程中释放。方法：测定糖尿病患者早餐前后β - 1- hdl前浓度、卵磷脂-胆固醇酰基转移酶（LCAT）活性和LCAT依赖性β - 1- hdl前转化。我们还使用tgrl进行了体外研究。采用酶联免疫吸附法和天然二维梯度凝胶（N-2D-gel）电泳定量前β1- hdl。结果：糖尿病组早餐前β1- hdl浓度高于健康对照组；早餐后，两组的水平相似。早餐后，LCAT质量和LCAT依赖的前β1- hdl转化率均未发生变化。空腹血浆与乳糜微粒或极低密度脂蛋白（VLDL）混合，分别使β前1- hdl水平降低15%±4%和45%±10%。n - 2d凝胶电泳显示，细菌来源的TG脂肪酶仅从2型糖尿病患者餐后VLDL中生成β1- hdl。结论：pre - β1- hdl在餐后状态与tgrl结合，并在脂解过程中释放，提示餐后高脂血症损害控制不良的2型糖尿病患者的逆向胆固醇转运。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Biochemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

5.20

自引率

4.50%

发文量

期刊介绍： Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine. Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals. Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).