Knockdown of RFC2 Prevents the Proliferation, Migration and Invasion of Cervical Cancer Cells.

IF 1.6 4区医学 Q4 ONCOLOGY

Anticancer research Pub Date : 2025-03-01 DOI:10.21873/anticanres.17486

Jae Woong Koh, Seon-Joo Park

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引用次数: 0

Abstract

Background/aim: Replication factor C subunit 2 (RFC2) is a component of the replication factor C (RFC) complex, which plays a critical role in DNA replication and repair. Recent studies have reported the involvement of RFC2 in several cancers. In this study, we investigated the functional role of RFC2 in cervical cancer progression.

Materials and methods: RFC2 expression in cervical cancer cells was analyzed using quantitative real-time PCR (qRT-PCR) and western blotting. HeLa, ME-180, and SiHa cells were transfected with siRNAs targeting RFC2. Cell viability and proliferation were assessed using the MTT and colony formation assays, and cell cycle distribution was analyzed by flow cytometry. Migration and invasion abilities were evaluated through Transwell assays with or without Matrigel.

Results: RFC2 knockdown significantly reduced cell proliferation in cervical cancer cells. Flow cytometry analysis revealed cell cycle arrest at the S phase. Additionally, RFC2 knockdown markedly inhibited the migration and invasion of cervical cancer cells. These results demonstrate the critical involvement of RFC2 in regulating proliferation and metastatic potential in cervical cancer cells.

Conclusion: RFC2 plays a pivotal role in promoting cervical cancer progression by enhancing cell growth, regulating the cell cycle, and promoting metastatic behavior. Further studies on the molecular mechanisms of RFC2 in cancer-associated pathways may provide a novel therapeutic target for developing cervical cancer treatment strategies.

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敲低RFC2抑制宫颈癌细胞增殖、迁移和侵袭。

背景/目的：复制因子C亚单位2 （RFC2）是复制因子C （RFC2）复合体的一个组成部分，在DNA复制和修复中起关键作用。最近的研究报道了RFC2与几种癌症有关。在这项研究中，我们研究了RFC2在宫颈癌进展中的功能作用。材料与方法：采用实时荧光定量PCR （qRT-PCR）和western blotting检测宫颈癌细胞中RFC2的表达。用靶向RFC2的sirna转染HeLa、ME-180和SiHa细胞。采用MTT法和集落形成法检测细胞活力和增殖，流式细胞术检测细胞周期分布。通过使用或不使用Matrigel的Transwell试验评估迁移和入侵能力。结果：RFC2基因敲除显著降低宫颈癌细胞增殖。流式细胞术分析显示细胞周期阻滞在S期。此外，RFC2敲低可显著抑制宫颈癌细胞的迁移和侵袭。这些结果证明了RFC2在调节宫颈癌细胞增殖和转移潜能中的重要作用。结论：RFC2通过促进细胞生长、调节细胞周期、促进转移行为，在促进宫颈癌进展中起关键作用。进一步研究RFC2在癌症相关通路中的分子机制，可能为制定宫颈癌治疗策略提供新的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.