Isolation and investigation of anti-tubercular ilicic acid from Sphaeranthus indicus against Mycobacterium tuberculosis H37Rv and MDR strains

IF 3 4区医学 Q1 MEDICINE, LEGAL

Regulatory Toxicology and Pharmacology Pub Date : 2025-03-03 DOI:10.1016/j.yrtph.2025.105800

Alex Yagoo , M.C. John Milton , Jelin Vilvest , A. Arokia Ahino Jessie , Kedike Balakrishna

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引用次数: 0

Abstract

The global burden of tuberculosis, particularly multidrug-resistant (MDR) strains of Mycobacterium tuberculosis, necessitates the urgent development of novel and effective therapeutic agents. Natural products derived from plants have long served as an essential resource for drug discovery, offering structurally diverse bioactive compounds. Sphaeranthus indicus, a plant traditionally valued for its medicinal properties, has shown promise as a source of antimicrobial agents. This study evaluated the antimycobacterial potential of S. indicus extracts and the isolated compound ilicic acid against M. tuberculosis H₃₇Rv and MDR isolates. Hexane, chloroform, and methanol extracts were screened using the microbroth dilution assay, with the hexane extract demonstrating superior activity (MIC: 125 μg/mL) against the H₃₇Rv strain. Purification of the hexane extract led to the isolation of ilicic acid, which exhibited significant antimycobacterial activity, inhibiting H₃₇Rv at 125 μg/mL. Against MDR isolates, ilicic acid displayed MIC values of 500 μg/mL for isolate 1, 125 μg/mL for isolate 2, and 250 μg/mL for isolate 3. These findings underscore the therapeutic potential of ilicic acid as a lead compound for developing anti-TB drugs, especially against drug-resistant strains. The study highlights S. indicus as a valuable source for discovering novel antimycobacterial agents, contributing to global efforts to combat TB resistance.

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来源期刊

Regulatory Toxicology and Pharmacology 医学-毒理学

CiteScore

6.70

自引率

8.80%

发文量

147

审稿时长

58 days

期刊介绍： Regulatory Toxicology and Pharmacology publishes peer reviewed articles that involve the generation, evaluation, and interpretation of experimental animal and human data that are of direct importance and relevance for regulatory authorities with respect to toxicological and pharmacological regulations in society. All peer-reviewed articles that are published should be devoted to improve the protection of human health and environment. Reviews and discussions are welcomed that address legal and/or regulatory decisions with respect to risk assessment and management of toxicological and pharmacological compounds on a scientific basis. It addresses an international readership of scientists, risk assessors and managers, and other professionals active in the field of human and environmental health. Types of peer-reviewed articles published: -Original research articles of relevance for regulatory aspects covering aspects including, but not limited to: 1.Factors influencing human sensitivity 2.Exposure science related to risk assessment 3.Alternative toxicological test methods 4.Frameworks for evaluation and integration of data in regulatory evaluations 5.Harmonization across regulatory agencies 6.Read-across methods and evaluations -Contemporary Reviews on policy related Research issues -Letters to the Editor -Guest Editorials (by Invitation)