Contrasting effects of prolonged drought and nitrogen addition on growth and non-structural carbohydrate dynamics in coexisting Pinus koraiensis and Fraxinus mandshurica saplings.

Abstract

Global change drivers, including drought and nitrogen (N) deposition, exert a wide-ranging influence on tree growth and fitness. However, our current understanding of their combined effects is still limited. Non-structural carbohydrate (NSC) storage is an important physiological trait for tree acclimation to drought. It acts as an important mobile carbon reserve to support tree function when carbon fixation or transport are reduced under drought. It is crucial to investigate how tree species with different NSC storage characteristics (e.g., storage level, partitioning) respond to drought events, and how N alters these patterns. We investigated the combined effects of drought (80% reduction in precipitation) and N addition (0, 30, and 120 kg/ha/year) on the growth and NSC storage of Pinus koraiensis and Fraxinus mandshurica (dominant species in the forests of Northeast China) saplings over two consecutive growing seasons. The results indicated that P. koraiensis exhibited high tolerance to drought, with growth unaffected by drought alone until the mid-growing season in the second year. However, N addition reversed its drought acclimation by impairing root development and exacerbating carbon shortage. In contrast, F. mandshurica was sensitive to drought, it had significantly reduced growth at harvest despite a large amount of NSC accumulation. The present study highlights the contrasting effects of N deposition on drought adaptation in coexisting conifer and temperate broadleaf species, the conifer showing a higher risk of carbon deficiency with increasing N deposition (i.e., a stronger reversal effect of N addition), whereas an earlier cessation of growth under drought defines a larger carbon safety margin for broadleaved species. These results have important implications for the development of adaptive forest management strategies such as to enhance the protection of conifers in the context of global change.