Evaluation of PI3K Levels and miRNA124-5p Expression Levels in Serum Samples from Patients With Lung Cancer.

Cancer diagnosis & prognosis Pub Date : 2025-03-03 eCollection Date: 2025-03-01 DOI:10.21873/cdp.10433

Fatma Tuba Akdeniz, Orcun Avsar

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Abstract

Background/aim: Lung cancers are malignant neoplasms located in the lung tissues. miRNAs are short non-coding RNAs. It is known that miRNA-124 prevents metastasis in lung cancers. The phosphatidylinositol 3-kinases (PI3K) signaling pathway, a basic signaling pathway interconnected with other pathways, is activated during cancer development. This study aimed to compare miRNA-124-5p and PI3K serum levels in patient and control groups.

Materials and methods: miRNA isolated from patient and control serum samples were converted into cDNA. miRNA-124-5p expression was determined using Real-Time PCR and a SYBR GREEN kit. PI3K serum level was determined using the Enzyme-Linked Immunosorbent Assay.

Results: While miRNA-124-5p serum level was statistically significantly lower in the patient group (p>0.02), serum PI3K level was higher in the patient group than in the control group but the difference was not statistically significant (p>0.11).

Conclusion: Lower serum levels of miRNA-124-5p and high PI3K levels observed in the patient group, compared to the control group, may be associated with a poor disease prognosis.

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肺癌患者血清样本中PI3K水平和miRNA124-5p表达水平的评估

背景/目的：肺癌是位于肺组织的恶性肿瘤。mirna是短的非编码rna。众所周知，miRNA-124可以阻止肺癌的转移。磷脂酰肌醇3-激酶（PI3K）信号通路是与其他信号通路相互关联的基本信号通路，在癌症发生过程中被激活。本研究旨在比较患者和对照组中miRNA-124-5p和PI3K的血清水平。材料和方法：从患者和对照血清中分离miRNA转化为cDNA。采用Real-Time PCR和SYBR GREEN试剂盒检测miRNA-124-5p的表达。采用酶联免疫吸附法测定血清PI3K水平。结果：患者组血清miRNA-124-5p水平低于对照组，差异有统计学意义（p>0.02）；患者组血清PI3K水平高于对照组，但差异无统计学意义（p>0.11）。结论：与对照组相比，患者组血清miRNA-124-5p水平较低，PI3K水平较高，可能与疾病预后较差有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Cancer diagnosis & prognosis

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