Nanosecond Pulsed Bipolar Cancellation of the Killing Effect on Glioblastoma.

IF 4.4 2区医学 Q2 ENGINEERING, BIOMEDICAL

IEEE Transactions on Biomedical Engineering Pub Date : 2025-02-28 DOI:10.1109/TBME.2025.3536477

Zhijun Luo, Fei Guo, Sizhe Xiang, Shoulong Dong, Chenguo Yao, Huawen Liu

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引用次数: 0

Abstract

Objective: Glioblastoma (GBM) is the deadliest type of cancer and current clinical treatments for malignant gliomas have many side effects. The article discusses the possibility that nanosecond pulsed electric fields (nsPEFs) can be focused on tumors for local killing. As well as the possibility of utilizing the CANCAN (canceled bipolar) effect to reduce neurostimulation and thus overcome side effects such as seizures and edema.

Method: In this paper, we use cell ablation and viability experiments to investigated the BPC (Bipolar cancellation) effect of U87-MG cells under the action of nsPEFs of various pulse numbers and the electric field amplitude.

Results: The results showed that maximum BPC efficiency (163.9%) was obtained with nsPEFs of 15 kV/cm and 15 pulses, and unipolar nsPEFs of 20 kV/cm and 15 pulses were able to achieve a killing effect of 90% with cell suspension, then this electric field is used as a reference for the ablation experiments.

Conclusion: Cell ablation experiments found that the electric field threshold of 3D (3D-like tissue) cell ablation (5.805 ± 1.455 kV/cm) is lower than that of monolayer wall cells (8.95 ± 0.75 kV/cm), which can cause a larger ablation area under the same pulsed electric field conditions. In addition, the BPC effect was more significant for 3D cells, but the trends of ablation area and BPC efficiency were similar when modulating the number of pulses.

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纳秒脉冲双极对消对胶质母细胞瘤的杀伤作用。

目的：胶质母细胞瘤（GBM）是最致命的癌症类型，目前临床治疗恶性胶质瘤的方法有很多副作用。文章讨论了纳秒脉冲电场（nsPEFs）聚焦于肿瘤进行局部杀伤的可能性。以及利用 CANCAN（取消双极）效应减少神经刺激，从而克服癫痫发作和水肿等副作用的可能性：本文利用细胞消融和存活实验研究了 U87-MG 细胞在不同脉冲数和电场幅值的 nsPEF 作用下的 BPC（双极消除）效应：结果表明，15千伏/厘米、15个脉冲的nsPEF获得了最大的BPC效率（163.9%），20千伏/厘米、15个脉冲的单极nsPEF对细胞悬浮液的杀伤效果达到了90%，然后以此电场作为消融实验的参考：细胞消融实验发现，三维（三维类组织）细胞消融的电场阈值（5.805 ± 1.455 kV/cm）低于单层壁细胞消融的电场阈值（8.95 ± 0.75 kV/cm），在相同的脉冲电场条件下，三维（三维类组织）细胞消融的电场阈值可使消融面积更大。此外，三维细胞的 BPC 效应更为显著，但在调节脉冲数时，消融面积和 BPC 效率的趋势相似。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

IEEE Transactions on Biomedical Engineering 工程技术-工程：生物医学

CiteScore

9.40

自引率

4.30%

发文量

880

审稿时长

2.5 months

期刊介绍： IEEE Transactions on Biomedical Engineering contains basic and applied papers dealing with biomedical engineering. Papers range from engineering development in methods and techniques with biomedical applications to experimental and clinical investigations with engineering contributions.