X-chromosome-linked miR-542-5p as a key regulator of sex disparity in rats with adjuvant-induced arthritis by promoting Th17 differentiation.

IF 9.5 2区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Biomarker Research Pub Date : 2025-03-01 DOI:10.1186/s40364-025-00741-x

Jiu Jie Yang, Zhi Li, Lin Na Wang, Bai Xiong Huang, Jerome P L Ng, Xiong Fei Xu, Yu Ping Wang, David Wei Zhang, Bo Qin, Ding Qi Zhang, Chang Liu, Wei Dan Luo, Betty Yuen Kwan Law, Hui Miao Wang, Meng Han Liu, Xiao Yun Yun, Joyce Tsz Wai Chan, Wan Yu Wu, Yi Ting Li, Peter Kam Fai Cheung, Man Chon Pou, Kat Sang Ha, Wang Fai Ao Ieong, Chi Hou Leong, Kit Ieng Leong, Chan Wang Lei, Lek Hang Cheang, Vincent Kam Wai Wong

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引用次数: 0

Abstract

Background: Studies have indicated that X-linked microRNAs (miRNAs) play a role in the pathogenesis of rheumatoid arthritis (RA) and its gender-specific differences. However, research on specific miRNAs remains limited. This study aims to investigate the possible role of X-linked miR-542-5p in RA pathogenesis and gender differences.

Methods: We investigated the impact of miR-542-5p on RA pathogenesis and gender differences by manipulating its expression in various rat models.

Results: Our findings revealed a significant overexpression of miR-542-5p in RA patients compared with healthy individuals, with a notable gender difference among RA patients. In vivo experiments confirmed that upregulation of miR-542-5p could accelerate RA pathogenesis. Further analysis showed that the onset of adjuvant-induced arthritis (AIA) in rats exhibited significant gender differences, with more severe clinical phenotypes found in female rats. This may be attributed to their stronger immune responses and elevated levels of miR-542-5p. Subsequent in vitro and in vivo experiments demonstrated that miR-542-5p contributes to the regulation of gender differences in RA pathogenesis by promoting the differentiation of Th17 cells.

Conclusions: This study offers new insights into the sex-specific nature of RA, suggesting X-linked miR-542-5p as a potential target for both diagnostic and therapeutic purposes. These findings lay the groundwork for the development of gender-specific therapeutic strategies for RA and underscore the importance of gender consideration in RA research.

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来源期刊

Biomarker Research Biochemistry, Genetics and Molecular Biology-Molecular Medicine

CiteScore

15.80

自引率

1.80%

发文量

审稿时长

10 weeks

期刊介绍： Biomarker Research, an open-access, peer-reviewed journal, covers all aspects of biomarker investigation. It seeks to publish original discoveries, novel concepts, commentaries, and reviews across various biomedical disciplines. The field of biomarker research has progressed significantly with the rise of personalized medicine and individual health. Biomarkers play a crucial role in drug discovery and development, as well as in disease diagnosis, treatment, prognosis, and prevention, particularly in the genome era.