Exploring Crocin's Role in Alleviating Memory Impairments and Depression-like Behaviors Induced by REM Sleep Deprivation, Focusing on BDNF and GSK-3β in Male Rats.

IF 4.6 2区医学 Q1 NEUROSCIENCES

Molecular Neurobiology Pub Date : 2025-07-01 Epub Date: 2025-03-03 DOI:10.1007/s12035-025-04753-4

Golbahar Lotfi, Maryam Eslami, Mahdieh Gholami, Salar Vaseghi, Batool Ghorbani Yekta

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引用次数: 0

Abstract

Rapid-eye movement sleep deprivation (REM SD) drastically alters behavioral functions and the mood state. Evidence demonstrates that the effects of REM SD on cognition and mood vary significantly based on the duration of deprivation. On the other hand, crocin (as a natural therapeutic compound found in Saffron) may be beneficial for the alleviation of SD effect. The present research aimed to explore the effects of REM SD of varying durations on behavioral functions and the expression levels of brain-derived neurotrophic factor (BDNF) and glycogen synthase kinase-3 beta (GSK-3β) in the prefrontal cortex. REM SD was conducted for 1, 5, or 10 days (6 h per day). Crocin was injected intraperitoneally at the dose of 50 mg/kg. The results showed that REM SD for 5- and 10-days increased locomotor activity and rearing, decreased pain threshold, and impaired passive avoidance memory performance. REM SD for 5- and 10-days increased the expression levels of GSK-3β, while only REM SD for 5 days decreased BDNF levels. Crocin (only in rats exposed to REM SD for 5 days) restored behavioral alterations, while in rats exposed to REM SD for 10 days had no effect. Interestingly, crocin in rats exposed to REM SD for 5- and 10-days attenuated GSK-3β upregulation. In addition, crocin in rats exposed to REM SD for 5 but not 10 days attenuated BDNF down regulation. In conclusion, the duration of REM SD is a crucial determinant of its impact on cognitive and mood functions.

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探讨藏红花素在缓解快速眼动睡眠剥夺引起的记忆障碍和抑郁样行为中的作用，重点关注雄性大鼠BDNF和GSK-3β。

快速眼动睡眠剥夺（REM SD）会极大地改变行为功能和情绪状态。有证据表明，快速眼动睡眠对认知和情绪的影响因剥夺睡眠时间的长短而有显著差异。另一方面，藏红花素（在藏红花中发现的一种天然治疗化合物）可能有助于缓解SD效应。本研究旨在探讨不同持续时间的快速眼动SD对行为功能及脑源性神经营养因子（BDNF）和糖原合成酶激酶-3β (GSK-3β)在前额叶皮层表达水平的影响。REM SD分别进行1、5、10天（每天6小时）。腹腔注射藏红花素50 mg/kg。结果显示，5天和10天的快速眼动SD使运动活动和饲养能力增加，疼痛阈值降低，被动回避记忆能力受损。5和10 d的REM SD使GSK-3β表达水平升高，而仅5 d的REM SD使BDNF表达水平降低。藏红花素（仅在暴露于快速眼动SD 5天的大鼠中）恢复了行为改变，而在暴露于快速眼动SD 10天的大鼠中没有效果。有趣的是，暴露于REM SD 5天和10天的大鼠中，藏红花素减弱了GSK-3β的上调。此外，暴露于REM SD 5天而不是10天的大鼠，藏红花素可以减弱BDNF的下调。总之，快速眼动SD的持续时间是其对认知和情绪功能影响的关键决定因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Neurobiology 医学-神经科学

CiteScore

9.00

自引率

2.00%

发文量

480

审稿时长

1 months

期刊介绍： Molecular Neurobiology is an exciting journal for neuroscientists needing to stay in close touch with progress at the forefront of molecular brain research today. It is an especially important periodical for graduate students and "postdocs," specifically designed to synthesize and critically assess research trends for all neuroscientists hoping to stay active at the cutting edge of this dramatically developing area. This journal has proven to be crucial in departmental libraries, serving as essential reading for every committed neuroscientist who is striving to keep abreast of all rapid developments in a forefront field. Most recent significant advances in experimental and clinical neuroscience have been occurring at the molecular level. Until now, there has been no journal devoted to looking closely at this fragmented literature in a critical, coherent fashion. Each submission is thoroughly analyzed by scientists and clinicians internationally renowned for their special competence in the areas treated.