TCF7 functions as a prognostic biomarker in bladder cancer by strengthening EMT and stemness associated with TGF-β/SMAD3 signaling.

IF 3.5 2区生物学 Q3 CELL BIOLOGY

Molecular and Cellular Biochemistry Pub Date : 2025-07-01 Epub Date: 2025-03-01 DOI:10.1007/s11010-025-05241-y

Congcong Zhang, Haiping Cai, Mengnan Ye, Le Wang, Weiwei Liu, Qun Huang, Xingsi Peng, Guanquan Mao, Qingling Zhang, Yan Mei

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引用次数: 0

Abstract

Bladder cancer (BC) is one of the top ten most common tumors, with recurrence and metastasis being major causes of mortality among patients. A high recurrence rate is a hallmark of BC. Epithelial-mesenchymal transition (EMT) plays a role in the formation of cancer stem cells, tumor metastasis, and immune evasion. In our preliminary research, single-cell sequencing identified TCF7 as a gene associated with EMT. However, its biological function and transcriptional regulation mechanisms in BC remain unclear. This study aims to investigate TCF7's role and regulatory mechanisms in BC progression. TCF7 is a critical transcription factor promoting BC progression. High TCF7 expression in BC is significantly linked to poor patient prognosis. We uncovered a novel mechanism by which TCF7 drives EMT and stemness in BC through transcriptional regulation of TGFBR1, impacting the TGF-β/SMAD3 pathway. These findings enhance our understanding of BC progression and offer potential strategies for diagnosis, treatment, and prognosis.

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TCF7通过增强与TGF-β/SMAD3信号相关的EMT和干性，在膀胱癌中发挥预后生物标志物的作用。

膀胱癌（膀胱癌）是十大最常见的肿瘤之一，复发和转移是患者死亡的主要原因。高复发率是BC的标志。上皮间充质转化（Epithelial-mesenchymal transition， EMT）在肿瘤干细胞的形成、肿瘤转移和免疫逃避中起着重要作用。在我们的初步研究中，单细胞测序鉴定TCF7是一个与EMT相关的基因。然而，其在BC中的生物学功能和转录调控机制尚不清楚。本研究旨在探讨TCF7在BC进展中的作用和调控机制。TCF7是促进BC进展的关键转录因子。TCF7在BC中的高表达与患者预后不良显著相关。我们发现了一种新的机制，TCF7通过转录调控TGFBR1驱动BC的EMT和干细胞，影响TGF-β/SMAD3通路。这些发现增强了我们对BC进展的理解，并为诊断、治疗和预后提供了潜在的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and Cellular Biochemistry 生物-细胞生物学

CiteScore

8.30

自引率

2.30%

发文量

293

审稿时长

1.7 months

期刊介绍： Molecular and Cellular Biochemistry: An International Journal for Chemical Biology in Health and Disease publishes original research papers and short communications in all areas of the biochemical sciences, emphasizing novel findings relevant to the biochemical basis of cellular function and disease processes, as well as the mechanics of action of hormones and chemical agents. Coverage includes membrane transport, receptor mechanism, immune response, secretory processes, and cytoskeletal function, as well as biochemical structure-function relationships in the cell. In addition to the reports of original research, the journal publishes state of the art reviews. Specific subjects covered by Molecular and Cellular Biochemistry include cellular metabolism, cellular pathophysiology, enzymology, ion transport, lipid biochemistry, membrane biochemistry, molecular biology, nuclear structure and function, and protein chemistry.