Binding buffer optimization for TEOS-modified magnetite nanoparticle-based DNA extraction method

IF 4.3 3区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Materials Chemistry and Physics Pub Date : 2025-02-26 DOI:10.1016/j.matchemphys.2025.130622

Stanley Evander Emeltan Tjoa , Mudasir Mudasir , Edi Suharyadi , Budi Setiadi Daryono

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引用次数: 0

Abstract

Magnetic nanoparticle-based DNA extraction is a well-known method. In its application, TEOS is the most common component used as a coating agent. TEOS-modified magnetic nanoparticle (TMNP) was prepared using a ratio of 0.5 ml TEOS/g MNP. The resulting TMNP has a crystalline size of 19.8 nm and has ferromagnetic properties. Selection of the appropriate binding buffer with TMNP based on the response of DNA concentration, purity, and integrity was GuSCN at a concentration of 2.5 M and dissolved at pH 6.5. This combination of TMNP and binding buffer can extract DNA of various types of bacteria and produce DNA in the range of 2.55–12.45 μg and DNA purity at 260/280 is 1.58–2.27. the type of bacteria that can be isolated is Escherichia coli, Pseudomonas aeruginosa, Salmonella sp., Salmonella paratyphi, Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogenes, Shigella sonnei, Staphylococcus epidermidis, and Mycobacterium tuberculosis. The extracted DNA can also be used for probe-based and intercalating dye-based qPCR.

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来源期刊

Materials Chemistry and Physics 工程技术-材料科学：综合

CiteScore

8.70

自引率

4.30%

发文量

1515

审稿时长

69 days

期刊介绍： Materials Chemistry and Physics is devoted to short communications, full-length research papers and feature articles on interrelationships among structure, properties, processing and performance of materials. The Editors welcome manuscripts on thin films, surface and interface science, materials degradation and reliability, metallurgy, semiconductors and optoelectronic materials, fine ceramics, magnetics, superconductors, specialty polymers, nano-materials and composite materials.