Quercetin capped Au-Ag bimetallic nanoparticles for the detection of calcium dobesilate monohydrate in biological and environmental samples

IF 4 2区化学 Q2 CHEMISTRY, PHYSICAL

Journal of Molecular Structure Pub Date : 2025-02-20 DOI:10.1016/j.molstruc.2025.141808

Shaz Asrar , Amtul Qayoom , Saeeda Nadir Ali , Dilshad Hussain

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Abstract

The study details the synthesis of quercetin-capped Au-Ag bimetallic nanoparticles (Qct-AuAg BMNps) in an aqueous environment. UV visible spectra showed absorbance peak of gold and bimetallic nanoparticles at 524 nm and 403 nm, respectively. The SEM analysis of Qct-AuAg BMNps showed an average particle size of 67.5 nm with good uniformity (PdI: 0.386). FTIR confirmed the presence of respective functional groups in Qct-AuAg BMNps. The Qct-AuAg BMNps were used as a colorimetric sensor for detecting calcium dobesilate monohydrate (CDM) in aqueous solutions, showing a linear spectrophotometric relationship with CDM concentration ranging from 0.000001 mM to 10 mM (R² = 0.9984). The detection and quantification limits were 1.66 × 10⁻⁶ and 2.71 × 10⁻⁵ mM, respectively. The binding constant (K_a) was 9.8 × 10⁴ M⁻¹, with a 1:1 stoichiometry confirmed by Job's plot. The Qct-AuAg BMNps were successfully used to detect CDM in tap water and serum samples. The synthesis process was optimized using Box Behnken Design, with the optimal conditions found at 50 °C, 2 mM quercetin, and a 5:1 silver-to-gold ratio. Therefore, the synthesized quercetin capped gold silver bimetallic nanoparticles were proved effective for the detection of CDM in liquid samples.

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本研究详细介绍了在水环境中合成槲皮素封端的金银双金属纳米粒子（Qct-AuAg BMNps）。紫外可见光谱显示，金和双金属纳米粒子的吸光度峰值分别为 524 纳米和 403 纳米。Qct-AuAg BMNps 的扫描电子显微镜分析表明，其平均粒径为 67.5 纳米，具有良好的均匀性（PdI：0.386）。傅立叶变换红外光谱证实了 Qct-AuAg BMNps 中存在各自的官能团。将 Qct-AuAg BMNps 用作比色传感器检测水溶液中的一水多贝西酸钙（CDM），其与 CDM 浓度在 0.000001 mM 至 10 mM 之间呈线性分光光度关系（R² = 0.9984）。检测限和定量限分别为 1.66 × 10-⁶ 和 2.71 × 10-⁵ mM。结合常数（Ka）为 9.8 × 10⁴ M-¹，约伯图证实其化学计量为 1:1。Qct-AuAg BMNps 成功用于检测自来水和血清样品中的 CDM。使用盒式贝肯设计法对合成过程进行了优化，发现最佳条件为 50 °C、2 mM 槲皮素和 5:1 的银-金比例。因此，合成的槲皮素封端的金银双金属纳米粒子被证明可有效检测液体样品中的 CDM。

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来源期刊

Journal of Molecular Structure 化学-物理化学

CiteScore

7.10

自引率

15.80%

发文量

2384

审稿时长

45 days

期刊介绍： The Journal of Molecular Structure is dedicated to the publication of full-length articles and review papers, providing important new structural information on all types of chemical species including: • Stable and unstable molecules in all types of environments (vapour, molecular beam, liquid, solution, liquid crystal, solid state, matrix-isolated, surface-absorbed etc.) • Chemical intermediates • Molecules in excited states • Biological molecules • Polymers. The methods used may include any combination of spectroscopic and non-spectroscopic techniques, for example: • Infrared spectroscopy (mid, far, near) • Raman spectroscopy and non-linear Raman methods (CARS, etc.) • Electronic absorption spectroscopy • Optical rotatory dispersion and circular dichroism • Fluorescence and phosphorescence techniques • Electron spectroscopies (PES, XPS), EXAFS, etc. • Microwave spectroscopy • Electron diffraction • NMR and ESR spectroscopies • Mössbauer spectroscopy • X-ray crystallography • Charge Density Analyses • Computational Studies (supplementing experimental methods) We encourage publications combining theoretical and experimental approaches. The structural insights gained by the studies should be correlated with the properties, activity and/ or reactivity of the molecule under investigation and the relevance of this molecule and its implications should be discussed.