Sinhyotaklisan alleviates inflammation in LPS-activated macrophages by modulating the heme oxygenase pathway

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2025-02-25 DOI:10.1016/j.jep.2025.119548

Jung-Yun Ahn , Dong-Woo Lim , Sung Yun Park, Ju-Hee Lee

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引用次数: 0

Abstract

Ethnopharmacological relevance

Shinhyotaklisan (SHTLS) is a time-honored traditional Korean herbal formula composed of four key herbs: Lonicerae Flos, Astragali Radix, Angelicae Gigantis Radix, and Glycyrrhizae Radix et Rhizoma. It has been extensively used to treat inflammatory diseases by expelling excessive heat, detoxifying the body, and promoting the drainage of pus from abscesses.

Aim of the study

This study examines the therapeutic effects of SHTLS and elucidates its mechanisms of action in alleviating lipopolysaccharide (LPS)-induced inflammation in vitro.

Materials and methods

SHTLS was prepared by boiling four herbs in 30% ethanol, and its antioxidant and antimicrobial effects were assessed. Furthermore, SHTLS was applied to LPS-exposed RAW 264.7 cells, and its anti-inflammatory effects were evaluated using an MTT assay, nitric oxide (NO) and reactive oxygen species (ROS) assays, enzyme-linked immunosorbent assay, and western blotting.

Results

SHTLS demonstrated potent antioxidant and antimicrobial effects. It effectively suppressed LPS-induced inflammatory cascades, resulting in a significant reduction in pro-inflammatory cytokines, including IL-6 and TNF-α, in murine macrophage cells. Moreover, SHTLS decreased COX-2 and iNOS expression levels, primarily through the inhibition of NF-κB and MAPK signaling pathways. Additionally, SHTLS significantly reduced intracellular ROS and reactive nitrogen species levels by upregulating heme oxygenase-1 (HO-1) expression. The anti-inflammatory effects of SHTLS were diminished by the addition of an HO-1 inhibitor, underscoring its strong association with intracellular antioxidant mechanisms.

Conclusion

Our results suggest that SHTLS exhibits strong antioxidant and anti-inflammatory effects, primarily by upregulating HO-1 and inhibiting the NF-κB and MAPK pathways, highlighting its potential as a therapeutic agent for inflammation-related conditions.

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.