Correction to “Construction of competitive endogenous RNA network and identification of potential regulatory axis in vascular calcification” Xu, T, Li, Y, Cheng, M, et al. Construction of competitive endogenous RNA network and identification of potential regulatory axis in vascular calcification. FASEB J. 2024 Oct 31; 38(20):e70114. doi: 10.1096/fj.202400973RR

Abstract

In the original publication of the article, the authors checked the results and found a mistake in Figure 7B. The DAPI panel in the N group of original Figure 7B did not align with the merged panel in the group. Hence, they updated the figure. The DAPI panel in the revised figure 7B aligns with the merged panel.

The authors apologize for this error.

The corrected Figure 7 is as follows:

Figure 7 miR-145-5p overexpression inhibits VSMC calcification. (A) qRT-PCR analysis of Smad3 and miR-145-5p expression in VSMCs between normal and high phosphorus group; normalized by GAPDH or U6 respectively, *p < .05. (B) Immunofluorescence staining for Smad3 in VSMCs untreated or treated with β-GP. DAPI, staining for nucleus. Scale bars: 20 μm. (C,D) Alizarin red S staining in β-GP treated VSMC transferred with miR-145-5p mimic or miR-145-5p inhibitor compared with β-GP + NC1 or β-GP + NC2, *p < .05. (E,F) Western blot analyses of Smad3, osteoblast-specific marker (OPN), VSMC-specific marker (22α) in miR-145-5p mimic group or miR-145-5p inhibitor group compared with negative control group (β-GP + NC1; β-GP + NC2), *p < 0.05. (G) qRT-PCR analysis of Smad3 expression after transfection of miR-145-5p mimic or miR-145-5p inhibitor in high phosphorus model; compared with negative control group (β-GP + NC1; β-GP + NC2), *p < .05. (H) Sequence of the 3 -UTR of Smad3 mRNA that matches the miR-145-5p seed sequence. (I) Verification of Smad3 as a target gene of miR-145-5p utilizing the dual-luciferase reporter assay. Abbreviations: qRT-PCR, quantitative reverse transcription-polymerase chain reaction; VSMCs, vascular smooth muscle cells; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; DAPI, 4′,6-diamidino-2-phenylindole; Smad3, SMAD family member 3; OPN, Osteopontin; 22α, smooth muscle 22 alpha. ns: no significance. WT: wild type; MUT: mutant.