Capillary basal lamina in human brain and spinal cord has fibrillar collagen type I and type III: Ignorance may not be bliss.

Q3 Medicine
Free neuropathology Pub Date : 2025-02-18 eCollection Date: 2025-01-01 DOI:10.17879/freeneuropathology-2025-6159
Wen-Lang Lin, Dennis W Dickson
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引用次数: 0

Abstract

The capillary basal lamina (BL) located between the endothelial cell, pericyte and perivascular astrocyte plays important roles in normal and diseased central nervous system (CNS). Using immunohistochemistry (IHC), electron microscopy (EM) and post-embedding immunogold EM (IEM), we studied capillary BL in biopsy and autopsy tissues of human CNS from cases with and without significant brain pathology and aged from 4 days to 49 years. In all cases, IHC showed, in the BL of microvessels, immunoreactivity for collagen types I, III, IV, VI and fibronectin. EM revealed fusion of the BL of capillary endothelial cells or pericyte with perivascular astrocyte BL, which was focally split, resulting in expanded spaces bordered by BL and containing striated fibrils. There was no significant thickening of fused or split BL. IEM showed localization of collagen I and III to banded fibrils, and of collagen IV to split and fused BL. These characteristic ultrastructural findings in human capillary BL were not found in normal or transgenic mice. Our observations of fibrillar collagen in young individuals complement previous observations of similar findings in older individuals. This raises the possibility that fibrillar collagen in human vascular BL plays a significant role in CNS capillary physiology and pathophysiology. The species-specific differences in capillary morphology between humans and mice might have relevance to poor correlations between benefits of immunotherapy and drug treatment in mice compared with human.

人类大脑和脊髓的毛细血管基底层有1型和3型纤维性胶原蛋白:无知可能不是福。
毛细血管基底层(BL)位于内皮细胞、周细胞和血管周围星形胶质细胞之间,在正常和病变中枢神经系统(CNS)中起重要作用。采用免疫组织化学(IHC)、电子显微镜(EM)和免疫金包埋后电镜(IEM)技术,研究了年龄从4天到49岁的有或无明显脑病理的人中枢神经系统活检和尸检组织中的毛细血管BL。在所有病例中,免疫组化均显示微血管BL中I、III、IV、VI型胶原和纤维连接蛋白的免疫反应性。EM显示毛细血管内皮细胞或周细胞的BL与血管周围星形胶质细胞的BL融合,后者局部分裂,导致以BL为边界的空间扩大,含有条纹原纤维。融合或分裂的BL没有明显的增厚。IEM显示胶原I和III定位于带状原纤维,胶原IV定位于分裂和融合的BL,这些在正常或转基因小鼠中没有发现的人类毛细血管BL的超微结构特征。我们在年轻人中观察到的纤维胶原蛋白补充了先前在老年人中观察到的类似发现。这提示了人血管BL中纤维性胶原蛋白在中枢神经系统毛细血管生理和病理生理中发挥重要作用的可能性。人类和小鼠毛细血管形态的物种特异性差异可能与小鼠免疫治疗和药物治疗的益处与人类相比相关性较差有关。
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来源期刊
CiteScore
2.80
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0.00%
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3 weeks
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