Genomic and pathogenicity analyses to identify the causative agent from multiple serogroups of non-O1, non-O139 Vibrio cholerae in foodborne outbreaks.
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Abstract
In 2013, foodborne outbreaks in Japan were linked to non-O1, non-O139 Vibrio cholerae. However, laboratory tests have detected several serogroups, making it difficult to determine the causative agent. Therefore, whole-genome analyses revealed that only serogroup O144 V. cholerae possesses a genomic island with a type III secretion system (T3SS). A T3SS-deficient mutant was subsequently generated, and its pathogenicity was assessed using a rabbit ileal loop test. This led to the conclusion that serogroup O144 V. cholerae with T3SS was the causative agent of foodborne outbreaks. This study provides an illustrative example of the utilization of whole-genome data for pathogenicity and molecular epidemiological analyses in outbreak investigations.
期刊介绍：
Microbial Genomics (MGen) is a fully open access, mandatory open data and peer-reviewed journal publishing high-profile original research on archaea, bacteria, microbial eukaryotes and viruses.
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