Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

Abstract

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:

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  Separation of trehalulose fraction
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  Lyophilization
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  Confirmation of trehalulose

The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.