Extracellular metabolites relieve the inhibitory effect of ferrous ions on microbial reduction of schwertmannite

IF 3.6 2区地球科学 Q1 GEOCHEMISTRY & GEOPHYSICS

Chemical Geology Pub Date : 2025-02-25 DOI:10.1016/j.chemgeo.2025.122709

Changdong Ke , Qian Yao , Yanping Deng , Siyu Zhang , Zhiliang Li , Renren Wu , Chuling Guo , Zhi Dang

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引用次数: 0

Abstract

In the environment, microbe-mineral-metabolites are tightly linked. Currently, the microbe-mineral interactions have been studied, but the effect of microbial metabolites on the microbe-mineral interactions is unclear. This study investigated the effect of metabolites of Shewanella oneidensis (an iron-reducing bacterium, FeRB) (or Desulfosporosinus meridiei [a sulfate-reducing bacterium, SRB]) on SRB (or FeRB) mediated transformation of schwertmannite (Sch), a mineral in acidic soils rich in sulfate (SO₄²⁻). The results showed that microbial metabolites relieved the inhibition of FeRB and SRB by complexing ferrous ions (Fe²⁺), thus promoting the microbial reduction of Sch. Besides, metabolites altered the pathways of microbial reduction and mineral transformation. In the absence of metabolites, FeRB mediated Fe(III) reduction mainly by increasing the cell abundance, while in the presence of metabolites, FeRB upregulated gene expression (mtrC and omcA) to enhance the Fe(III) reduction, and the Sch transformation pathway was Sch → goethite → siderite. For SRB, metabolites upregulated the dsr gene expression to accelerate SRB reducing SO₄²⁻, and the Sch transformation pathway was Sch → goethite and mackinawite → siderite, mackinawite and pyrite. Metabolomics and high-performance liquid chromatography analysis revealed a large amount of phenylalanine (∼1.5 mM) and citric acid (∼0.2 mM) were present in the metabolites of FeRB and SRB, respectively, which complexed Fe²⁺ and promoted the microbial reduction of Sch. This study highlighted the important role of metabolites in maintaining soil biological activity and provided new insights into the microbe-mineral-metabolite interactions.

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来源期刊

Chemical Geology 地学-地球化学与地球物理

CiteScore

7.20

自引率

10.30%

发文量

374

审稿时长

3.6 months

期刊介绍： Chemical Geology is an international journal that publishes original research papers on isotopic and elemental geochemistry, geochronology and cosmochemistry. The Journal focuses on chemical processes in igneous, metamorphic, and sedimentary petrology, low- and high-temperature aqueous solutions, biogeochemistry, the environment and cosmochemistry. Papers that are field, experimentally, or computationally based are appropriate if they are of broad international interest. The Journal generally does not publish papers that are primarily of regional or local interest, or which are primarily focused on remediation and applied geochemistry. The Journal also welcomes innovative papers dealing with significant analytical advances that are of wide interest in the community and extend significantly beyond the scope of what would be included in the methods section of a standard research paper.