Molecular regulation of cardiomyocyte functions by exogenous hydrogen sulphide in Atlantic salmon (Salmo salar)

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-03-01 DOI:10.1016/j.ygeno.2025.111017

Carlo C. Lazado , Thinh Hoang Nhan , Vibeke Voldvik , Erik Burgerhout , Arvind Y.M. Sundaram , Torstein Tengs , Tone-Kari K. Østbye , Øivind Andersen

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引用次数: 0

Abstract

Hydrogen sulphide (H₂S) is known to regulate various physiological processes, but its role in fish cardiac function, especially at the molecular level, is poorly understood. This study examined the molecular functions of exogenous H₂S, using sodium hydrosulphide (NaHS) as a donor, on Atlantic salmon cardiomyocytes. NaHS concentrations of 10 to 160 μM showed limited cytotoxicity and no impact on cell proliferation, though higher doses increased ATP activity. Menadione and NaHS administered separately or sequentially differentially regulated the expression of antioxidant response and sulphide detoxification genes. Transcriptomic analysis over 24, 48, 72, and 120 h revealed differential gene expression related to metabolic recovery. Enriched Gene Ontology terms at 24 h included processes like cell signalling and lipid metabolism, shifting to lipid metabolism and ribosomal processes by 48 h. By 120 h, xenobiotic metabolism and RNA synthesis were prominent. The study highlights NaHS-induced metabolic adjustments, particularly in lipid metabolism, in Atlantic salmon cardiomyocytes.

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外源性硫化氢对大西洋鲑鱼心肌细胞功能的分子调控

众所周知，硫化氢（H2S）调节多种生理过程，但其在鱼类心脏功能中的作用，特别是在分子水平上的作用，尚不清楚。本研究以氢硫化钠（NaHS）为供体，研究了外源性H2S对大西洋鲑鱼心肌细胞的分子功能。NaHS浓度为10 ~ 160 μM时，细胞毒性有限，对细胞增殖无影响，但高剂量可增加ATP活性。美那酮和NaHS分别或顺序给药对抗氧化反应和硫化物解毒基因的表达有差异。24、48、72和120 h的转录组学分析揭示了与代谢恢复相关的差异基因表达。24 h时富集的基因本体术语包括细胞信号传导和脂质代谢等过程，48 h时转向脂质代谢和核糖体过程。到120 h时，外源代谢和RNA合成突出。该研究强调了nahs在大西洋鲑鱼心肌细胞中诱导的代谢调节，特别是脂质代谢。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.