Effect of Metformin on Spermatogenesis Restoration Following Spermatogonial Stem Cell Transplantation in An Azoospermia Mouse Model.

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Azim Hedayatpour, Peyman Modarresi, Shokoofeh Kazemzadeh, Alieh Bashghareh
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引用次数: 0

Abstract

Objective: Spermatogonial stem cell transplantation (SSCT) could be a helpful strategy for fertility restoration in patients with childhood cancer. Additionally, using metformin as an antioxidant may help mitigate damage caused by chemotherapy. This study aimed to evaluate the protective effects of metformin against oxidative stress caused by busulfan and cadmiuminduced damage while examining its role in enhancing spermatogenesis restoration following SSCT.

Materials and methods: In this experimental study, a long-term infertility mouse model was created using cadmium and busulfan treatment (BC, n=10). Cryopreserved SSCs were transplanted either alone (cryo+SSCT, n=10) or in combination with metformin (cryo+MET+SSCT, n=10). These experimental groups were compared to a control group (n=10). Flow cytometry was used to assess the protective effect of metformin against reactive oxygen species (ROS) production, and immunofluorescence evaluated proliferation and differentiation markers.

Results: The results of our study showed that ROS production decreased significantly in the cryopreservation group with metformin (P<0.05). The expression of proliferation and differentiation markers after transplantation was significantly increased in the cryopreservation group with metformin compared to the cryopreservation group containing a basic freezing medium (P<0.05).

Conclusion: Transplantation of SSCs combined with metformin significantly enhances spermatogenesis and improves the homing efficiency of transplanted SSCs. This approach holds great potential for restoring fertility in clinical settings, particularly in childhood cancer survivors.

二甲双胍对无精子症小鼠精原干细胞移植后精子发生恢复的影响。
目的:精原干细胞移植(SSCT)可能是儿童癌症患者恢复生育能力的有效策略。此外,使用二甲双胍作为抗氧化剂可能有助于减轻化疗造成的损害。本研究旨在评估二甲双胍对丁硫芬和镉引起的氧化应激的保护作用,并研究其在SSCT后促进精子发生恢复的作用。材料和方法:本实验研究采用镉和丁硫丹处理(BC, n=10)建立长期不育小鼠模型。冷冻保存的SSCs单独移植(冷冻+SSCT, n=10)或与二甲双胍联合移植(冷冻+MET+SSCT, n=10)。各实验组与对照组进行比较(n=10)。采用流式细胞术评估二甲双胍对活性氧(ROS)产生的保护作用,免疫荧光评估增殖和分化标志物。结果:我们的研究结果显示,二甲双胍冷冻保存组的ROS产量明显下降(p结论:二甲双胍联合SSCs移植可显著促进精子发生,提高移植SSCs的归巢效率。这种方法在临床环境中,特别是在儿童癌症幸存者中,具有很大的恢复生育能力的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cell Journal
Cell Journal CELL BIOLOGY-
CiteScore
3.40
自引率
5.00%
发文量
0
审稿时长
12 months
期刊介绍: The “Cell Journal (Yakhteh)“, formerly published as “Yakhteh Medical Journal”, is a quarterly English publication of Royan Institute. This journal focuses on topics relevant to cellular and molecular scientific areas, besides other related fields. The Cell J has been certified by Ministry of Culture and Islamic Guidance in 1999 and was accredited as a scientific and research journal by HBI (Health and Biomedical Information) Journal Accreditation Commission in 2000 which is an open access journal.
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