Identification of potential genes associated with metastasis in osteosarcoma: an integrated bioinformatics analysis.

Q1 Medicine

MUSCULOSKELETAL SURGERY Pub Date : 2025-02-25 DOI:10.1007/s12306-025-00891-z

I G E Wiratnaya, M D Ismail, F Hasan

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引用次数: 0

Abstract

This study aims to identify the potential genes, pathways, and tumor immune microenvironment that might be involved in the metastasis process of osteosarcoma (OS). The GEO2R tool was deployed to screen two datasets obtained from the Gene Expression Omnibus (GEO) database (GSE87624 and GSE85537). Integrated bioinformatic analyses were then performed to investigate Gene Ontology, potential pathways, protein-protein network interaction, core hub genes, genetic alterations, and immune cell infiltration. The hub gene expression levels were validated utilizing another dataset (GSE14329) and patient prognosis was validated using the GDC-TARGET OS dataset. Our analysis identified 263 differentially expressed genes (DEGs), predominantly associated with the PI3K-AKT signaling pathway. Analysis using Cytoscape based on DEGs revealed five validated core hub genes including COL6A1, MMP2, POSTN, TAGLN, and THY1. Additionally, TAGLN and THY1 have a significant association (P = 0.008) (P = 0.03) with unfavorable outcomes in osteosarcoma patients. This study unveiled that TAGLN and THY1 were associated with metastasis and poor prognosis in OS.

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鉴定与骨肉瘤转移相关的潜在基因：综合生物信息学分析。

本研究旨在确定骨肉瘤（osteosarcoma， OS）转移过程中可能涉及的潜在基因、途径和肿瘤免疫微环境。使用GEO2R工具筛选从Gene Expression Omnibus （GEO）数据库获得的两个数据集（GSE87624和GSE85537）。然后进行综合生物信息学分析，以研究基因本体，潜在途径，蛋白质-蛋白质网络相互作用，核心枢纽基因，遗传改变和免疫细胞浸润。中心基因表达水平使用另一个数据集（GSE14329）进行验证，患者预后使用GDC-TARGET OS数据集进行验证。我们的分析确定了263个差异表达基因（DEGs），主要与PI3K-AKT信号通路相关。基于DEGs的Cytoscape分析显示了五个验证的核心枢纽基因，包括COL6A1， MMP2， POSTN， TAGLN和THY1。此外，TAGLN和THY1与骨肉瘤患者的不良预后有显著相关性（P = 0.008）（P = 0.03）。本研究揭示了TAGLN和THY1与OS的转移和不良预后相关。

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来源期刊

MUSCULOSKELETAL SURGERY Medicine-Surgery

CiteScore

4.50

自引率

0.00%

发文量

期刊介绍： Musculoskeletal Surgery – Formerly La Chirurgia degli Organi di Movimento, founded in 1917 at the Istituto Ortopedico Rizzoli, is a peer-reviewed journal published three times a year. The journal provides up-to-date information to clinicians and scientists through the publication of original papers, reviews, case reports, and brief communications dealing with the pathogenesis and treatment of orthopaedic conditions.An electronic version is also available at http://www.springerlink.com.The journal is open for publication of supplements and for publishing abstracts of scientific meetings; conditions can be obtained from the Editors-in-Chief or the Publisher.