Detection of circulating immune complexes by Raji cell assay: comparison of flow cytometric and radiometric methods.

Diagnostic and clinical immunology Pub Date : 1988-01-01

S F Kingsmore, A D Crockard, A C Fay, T A McNeill, S D Roberts, J M Thompson

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Abstract

Several flow cytometric methods for the measurement of circulating immune complexes (CIC) have recently become available. We report a Raji cell flow cytometric assay (FCMA) that uses aggregated human globulin (AHG) as primary calibrator. Technical advantages of the Raji cell flow cytometric assay are discussed, and its clinical usefulness is evaluated in a method comparison study with the widely used Raji cell immunoradiometric assay. FCMA is more precise and has greater analytic sensitivity for AHG. Diagnostic sensitivity by the flow cytometric method is superior in systemic lupus erythematosus (SLE), rheumatoid arthritis, and vasculitis patients: however, diagnostic specificity is similar for both assays, but the reference interval of FCMA is narrower. Significant correlations were found between CIC levels obtained with both methods in SLE, rheumatoid arthritis, and vasculitis patients and in longitudinal studies of two patients with cerebral SLE. The Raji cell FCMA is recommended for measurement of CIC levels to clinical laboratories with access to a flow cytometer.

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Raji细胞法检测循环免疫复合物:流式细胞术与放射法的比较。

最近出现了几种用于循环免疫复合物(CIC)测量的流式细胞术方法。我们报告了Raji细胞流式细胞测定(FCMA)，使用聚集的人球蛋白(AHG)作为主要校准器。本文讨论了Raji细胞流式细胞术的技术优势，并与广泛使用的Raji细胞免疫放射测定法进行了方法比较，评价了其临床应用价值。FCMA对AHG的分析精度更高，分析灵敏度更高。流式细胞术在系统性红斑狼疮(SLE)、类风湿性关节炎和血管炎患者中的诊断敏感性更高:然而，两种方法的诊断特异性相似，但FCMA的参考区间更窄。两种方法在SLE、类风湿性关节炎和血管炎患者以及两例脑型SLE患者的纵向研究中均发现CIC水平显著相关。Raji细胞FCMA被推荐用于有流式细胞仪的临床实验室测量CIC水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Diagnostic and clinical immunology

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