SPOCD1 Enhances Cancer Cell Activities and Serves as a Prognosticator in Esophageal Squamous Cell Carcinoma.

IF 2.6 4区医学 Q2 GENETICS & HEREDITY

Cancer Genomics & Proteomics Pub Date : 2025-03-01 DOI:10.21873/cgp.20503

Tuvshin Bayasgalan, Mitsuro Kanda, Yusuke Sato, Haote Zhu, Mohammad Hussain Hamrah, Flor Esther Garza Martinez, Takahiro Shinozuka, Yuki Ito, Masahiro Sasahara, Dai Shimizu, Shinichi Umeda, Yoshikuni Inokawa, Norifumi Hattori, Masamichi Hayashi, Chie Tanaka, Yasuhiro Kodera

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引用次数: 0

Abstract

Background/aim: Comprehensive transcriptome analysis has revealed SPOC Domain Containing 1 (SPOCD1) as a potential biomarker for esophageal squamous cell carcinoma (ESCC). However, the expression and oncological roles of SPOCD1 in ESCC remains underexplored. We aimed to evaluate the role of SPOCD1 in oncogenesis and prognosis of ESCC in vitro and in vivoMaterials and Methods: The Cancer Cell Line Encyclopedia (CCLE) database was utilized to evaluate correlations between SPOCD1 expression and oncogenes in ESCC. mRNA and protein levels were measured by qRT-PCR and Simple Western assays, respectively. siRNA-mediated knockdown and overexpression experiments assessed the effects of SPOCD1 expression on proliferation, migration, and invasion of ESCC cell lines. In vivo, siRNA knockdown effects on tumor growth were tested in mouse xenograft models. SPOCD1 mRNA levels in 164 resected tissues were correlated with clinicopathological parameters and survival, while a cohort of 177 patients was analyzed for protein expression and survival.

Results: SPOCD1 mRNA expression varied widely among ESCC cell lines and correlated with epithelial-mesenchymal transition-related genes. Knockdown significantly suppressed proliferation, migration, and invasion (p<0.001), while overexpression increased proliferation (p<0.001). In vivo, siRNA knockdown reduced tumor growth compared to both si-control (p=0.005) and untransfected groups (p<0.001). High SPOCD1 mRNA expression was linked to poor disease-specific survival (p=0.009, HR=1.965, 95% CI=1.187-3.252) and disease-free survival (p=0.047, HR=1.602, 95% CI=1.007-2.549). Similarly, elevated protein levels were associated with unfavorable disease-specific (p=0.013, HR=1.860, 95% CI=1.137-3.041) and disease-free survival (p=0.032, HR=1.618, 95% CI=1.042-2.513).

Conclusion: SPOCD1 expression correlates with the aggressiveness of ESCC cells, and its expression levels in tumor tissues may serve as a prognostic factor for ESCC patients.

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SPOCD1增强癌细胞活性并作为食管癌的预后因子。

背景/目的：综合转录组分析显示，SPOC结构域1 （spod1）是食管鳞状细胞癌（ESCC）的潜在生物标志物。然而，SPOCD1在ESCC中的表达和肿瘤学作用仍未得到充分研究。我们的目的是评估SPOCD1在体外和体内ESCC的肿瘤发生和预后中的作用。材料和方法：利用Cancer Cell Line Encyclopedia （CCLE）数据库评估ESCC中SPOCD1表达与癌基因的相关性。分别用qRT-PCR和Simple Western法检测mRNA和蛋白水平。sirna介导的敲低和过表达实验评估了SPOCD1表达对ESCC细胞系增殖、迁移和侵袭的影响。在体内，在小鼠异种移植模型中测试了siRNA敲低对肿瘤生长的影响。164例切除组织中SPOCD1 mRNA水平与临床病理参数和生存率相关，同时对177例患者的队列进行蛋白表达和生存率分析。结果：SPOCD1 mRNA在ESCC细胞系中的表达差异较大，且与上皮-间质转化相关基因相关。在体内，与si对照组（p=0.005）和未转染组（pp=0.009， HR=1.965, 95% CI=1.187-3.252）相比，siRNA敲低可降低肿瘤生长（p=0.047， HR=1.602, 95% CI=1.007-2.549）和无病生存（p=0.047， HR=1.602, 95% CI=1.007-2.549）。同样，蛋白水平升高与不利的疾病特异性（p=0.013， HR=1.860, 95% CI=1.137-3.041）和无病生存（p=0.032， HR=1.618, 95% CI=1.042-2.513）相关。结论：SPOCD1的表达与ESCC细胞的侵袭性相关，其在肿瘤组织中的表达水平可能是影响ESCC患者预后的因素之一。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Genomics & Proteomics ONCOLOGY-GENETICS & HEREDITY

CiteScore

5.00

自引率

8.00%

发文量

期刊介绍： Cancer Genomics & Proteomics (CGP) is an international peer-reviewed journal designed to publish rapidly high quality articles and reviews on the application of genomic and proteomic technology to basic, experimental and clinical cancer research. In this site you may find information concerning the editorial board, editorial policy, issue contents, subscriptions, submission of manuscripts and advertising. The first issue of CGP circulated in January 2004. Cancer Genomics & Proteomics is a journal of the International Institute of Anticancer Research. From January 2013 CGP is converted to an online-only open access journal. Cancer Genomics & Proteomics supports (a) the aims and the research projects of the INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH and (b) the organization of the INTERNATIONAL CONFERENCES OF ANTICANCER RESEARCH.