Optimization of Chitosan-Based Film Performance by the Incorporation of Cinnamomum Zeylanicum Essential Oil

IF 2.8 4区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Biophysics Pub Date : 2025-02-25 DOI:10.1007/s11483-025-09943-0

Anouar Mouhoub, Amine Guendouz, Zainab El Alaoui-Talibi, Saad Ibnsouda Koraichi, Cherkaoui El Modafar

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Abstract

Lately, Chitosan-based films (C-films) incorporating essential oils (EOs) have gained researchers' attention due to their biodegradability and excellent bioactivities. Nevertheless, the EOs' high cost is considered a constraint. Therefore, we aimed to develop C-films enriched with different concentrations of Cinnamomum zeylanicum EO (0.5% to 8%) and evaluate their global performance. The incorporation of the EO was verified by Fourier transform infrared spectroscopy analysis. As regards the physicochemical and mechanical analysis, experimental data revealed a considerable augmentation in the C-film opacity (from 1.50 to 6.10), thickness (from 15 to 195 μm), and tensile strength (TS) (from 5.12 to 21.74 MPa) following the enrichment with EO. However, the treated C-films showed a decrease in moisture content (MC) (from 40.06 to 10.91%), swelling level (SL) (from 763.20 to 5.12%), hydrosolubility (HS) (from 63.26 to 51.28%), hydrophobicity (from θ_W = 98.06° to θ_W = 20.53°), elongation at break (EB) (from 159.12 to 16.97%), and water vapor transmission rate (WVTR) (from 71.05 to 10.50 g/h.m²). The variation in these parameters was proportional to the EO concentration. Concerning the biological activities, the C-film incorporating EO at 8% inhibited over 80% of the tested radicals, while the antioxidant activity of the control C-film was negligible. Furthermore, the C-film enriched with EO at 8% exhibited great antibacterial activity against Enterococcus hirae, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, and inhibited their biofilm development by more than 95%. Ultimately, molecular docking revealed a high affinity between EO major constituents and bacterial proteins involved in biofilm establishment (< -4.9 kcal/mol). These encouraging findings indicate that the elaborated C-films present remarkable potential for application in the food sector as an alternative to fossil-based packaging and synthetic agents.

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肉桂精油对壳聚糖基膜性能的优化

近年来，壳聚糖基精油膜（c -film）因其可生物降解性和良好的生物活性而受到研究人员的关注。然而，EOs的高成本被认为是一个限制。因此，我们的目标是制备富含不同浓度的肉桂EO（0.5% ~ 8%）的c膜，并评估其整体性能。傅里叶变换红外光谱分析验证了EO的引入。在理化和力学分析方面，实验数据显示，添加EO后，c膜的不透明度（从1.50增加到6.10）、厚度（从15增加到195 μm）和抗拉强度（TS）（从5.12增加到21.74 MPa）显著增加。处理后的c膜含水率（MC）从40.06降至10.91%，溶胀度（SL）从763.20降至5.12%，溶解度（HS）从63.26降至51.28%，疏水性（θW = 98.06°降至θW = 20.53°），断裂伸长率（EB）从159.12降至16.97%，水蒸气透过率（WVTR）从71.05降至10.50 g/h.m2。这些参数的变化与EO浓度成正比。在生物活性方面，加入8% EO的c膜抑制了80%以上的自由基，而对照c膜的抗氧化活性可以忽略不计。此外，含有8% EO的c膜对人肠球菌、大肠埃希菌、铜绿假单胞菌和金黄色葡萄球菌均表现出良好的抑菌活性，抑制其生物膜形成的作用超过95%。最终，分子对接揭示了EO主要成分与参与生物膜建立的细菌蛋白之间的高亲和力（< -4.9 kcal/mol）。这些令人鼓舞的发现表明，精心制作的c膜作为化石基包装和合成剂的替代品，在食品领域具有显著的应用潜力。

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来源期刊

Food Biophysics 工程技术-食品科技

CiteScore

5.80

自引率

3.30%

发文量

审稿时长

1 months

期刊介绍： Biophysical studies of foods and agricultural products involve research at the interface of chemistry, biology, and engineering, as well as the new interdisciplinary areas of materials science and nanotechnology. Such studies include but are certainly not limited to research in the following areas: the structure of food molecules, biopolymers, and biomaterials on the molecular, microscopic, and mesoscopic scales; the molecular basis of structure generation and maintenance in specific foods, feeds, food processing operations, and agricultural products; the mechanisms of microbial growth, death and antimicrobial action; structure/function relationships in food and agricultural biopolymers; novel biophysical techniques (spectroscopic, microscopic, thermal, rheological, etc.) for structural and dynamical characterization of food and agricultural materials and products; the properties of amorphous biomaterials and their influence on chemical reaction rate, microbial growth, or sensory properties; and molecular mechanisms of taste and smell. A hallmark of such research is a dependence on various methods of instrumental analysis that provide information on the molecular level, on various physical and chemical theories used to understand the interrelations among biological molecules, and an attempt to relate macroscopic chemical and physical properties and biological functions to the molecular structure and microscopic organization of the biological material.