{"title":"Calcium Imaging in Hypothalamic Cells.","authors":"Matei Bolborea","doi":"10.1007/978-1-0716-4284-9_15","DOIUrl":null,"url":null,"abstract":"<p><p>Calcium imaging is a powerful tool to explore communication in neural networks. The hypothalamus is the region of the brain that regulates functions such as reproduction, appetite, lactation, stress, or hydration. Understanding the orchestration of neural networks in this region is a fundamental question of neuroscience. Calcium imaging can be used and performed not only on dissociated cells and acute brain slices but also on living animals. To date, the ideal experimental setup to explore nutrient sensing in the hypothalamus is acute brain calcium imaging. The technique readily incorporates pharmacological tools, as it uses bath applications or patch puffing. The technique can also be coupled with electrophysiological recordings.Here is presented the technical protocol for calcium imaging on acute brain slices of mice hypothalami.</p>","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2882 ","pages":"305-316"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-1-0716-4284-9_15","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Calcium imaging is a powerful tool to explore communication in neural networks. The hypothalamus is the region of the brain that regulates functions such as reproduction, appetite, lactation, stress, or hydration. Understanding the orchestration of neural networks in this region is a fundamental question of neuroscience. Calcium imaging can be used and performed not only on dissociated cells and acute brain slices but also on living animals. To date, the ideal experimental setup to explore nutrient sensing in the hypothalamus is acute brain calcium imaging. The technique readily incorporates pharmacological tools, as it uses bath applications or patch puffing. The technique can also be coupled with electrophysiological recordings.Here is presented the technical protocol for calcium imaging on acute brain slices of mice hypothalami.
期刊介绍：
For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
