Anti-inflammatory and non-toxic effects of Doyerea emetocathartica aqueous extract in mice

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL

Journal of ethnopharmacology Pub Date : 2025-02-21 DOI:10.1016/j.jep.2025.119534

Genil Dantas de Oliveira , Mariana França de Moraes , Magna Maria Lima Araújo , Dilma Maria de Brito Melo Trovão , José Iranildo Miranda de Melo , Wêndeo Kennedy Costa , Maria Tereza dos Santos Correia , Alisson Macário de Oliveira , Samuel Paulo Cibulski , Harley da Silva Alves

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引用次数: 0

Abstract

Ethnopharmacological relevance

Doyerea emetocathartica is a Cucurbitaceae known as ''cabeça de negro'' and used by North-eastern Brazil and South-eastern Mexico communities to treat rheumatism, inflammation and pain. Despite ethnopharmacological reports, there is currently a lack of evidence about its chemical composition, toxicity and pharmacological effects.

Aim of the study

This study aimed to investigate the chemical composition of the aqueous extract of D. emetocathartica stems (DEAQ), evaluate its acute oral toxicity, mutagenicity and its anti-inflammatory profile in mice.

Material and methods

The DEAQ chemical composition was evaluated through HPLC-MS/MS. Acute oral toxicity was assessed for 14 days after the oral administration of DEAQ in a single dose of 2000 mg kg⁻¹. The mutagenic assessment was carried out through micronucleus test in mice. The DEAQ anti-inflammatory activity was evaluated at doses of 25, 50 and 100 mg kg⁻¹ throught models of carrageenan-induced paw edema, peritonitis and air pouch.

Results

Using HPLC-MS/MS the cayaponoside D₂ was identified as the most abundant compound in DEAQ. Oral DEAQ administration showed no signs of toxicity or mutagenicity. All tested DEAQ doses had anti-edematogenic effects, decreased the migration of total leukocytes and neutrophils and decreased the levels of TNF-α and IL-1β.

Conclusions

The in vivo tests results indicated that DEAQ showed strong anti-inflammatory activity, with possible cayaponoside D₂ involvement, highlighting the absence of toxicity and genotoxicity for the dose and route used. Furthermore, the study provides chemical and pharmacological evidences about the therapeutic use of D. emetocathartica in folk medicine.

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.