Investigation on the mechanism of simulated microgravity promoting rat cerebral angiogenesis based on proteomics

IF 3.1 2区物理与天体物理 Q1 ENGINEERING, AEROSPACE

Acta Astronautica Pub Date : 2025-02-14 DOI:10.1016/j.actaastro.2025.02.024

Yaoyuan Cui , Huayan Liu , Zhuohan Li , Bin Luo , Juan Zhao , Shiyi Tang , Anqing Wang , Guan Wang , Jichen Du , Jing Yang

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引用次数: 0

Abstract

Changed neurovascular homeostasis under microgravity may be closely related to the dysregulated angiogenesis resulting in neurovascular units and microvascular hyperpermeability. The present study aimed to investigate the mechanism of 7-day simulated microgravity (7d-SMG) promoting rat cerebral angiogenesis based on proteomics. 7d-SMG effect led to a slight inflammatory infiltration in rat cerebral cortex. The levels of oxidative stress, inflammatory factors, and pro-angiogenic factors were significantly increased. A total of 5499 proteins were detected by label-free quantitative proteomic strategy and 704 proteins were differentially expressed proteins (DEPs). 440 and 264 of DEPs were upregulated and downregulated, respectively. Gene-Ontology analysis suggested that most of DEPs were involved in brain development, actin cytoskeleton and filament organization, cell proliferation, which were closely associated with cerebral angiogenesis. The KEGG results showed that Rap1, Ras and other signaling pathways related to cell proliferation were significantly influenced by 7d-SMG. The expressions of VEGFR2, Crk, Rap1b, B-Raf, and the radio of pho-MEK/MEK, pho-ERK1/2/ERK1/2, Bcl2/Bax were respectively increased by 47.24 %, 25.11 %, 22.02 %, 41.44 %, 22.34 %, 24.87 %, 120.65 %, which indicated that 7d-SMG promoted cell proliferation through VEGFR2-Rap1b-ERK signaling pathway. The present study explored the effect of 7d-SMG regulating rat cerebral angiogenesis, which provided a novel insight to elucidate the potential mechanism of disrupted neurovascular homeostasis induced by MG. The expected results might benefit for maintaining nerve system health of astronauts during space flight.

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来源期刊

Acta Astronautica 工程技术-工程：宇航

CiteScore

7.20

自引率

22.90%

发文量

599

审稿时长

53 days

期刊介绍： Acta Astronautica is sponsored by the International Academy of Astronautics. Content is based on original contributions in all fields of basic, engineering, life and social space sciences and of space technology related to: The peaceful scientific exploration of space, Its exploitation for human welfare and progress, Conception, design, development and operation of space-borne and Earth-based systems, In addition to regular issues, the journal publishes selected proceedings of the annual International Astronautical Congress (IAC), transactions of the IAA and special issues on topics of current interest, such as microgravity, space station technology, geostationary orbits, and space economics. Other subject areas include satellite technology, space transportation and communications, space energy, power and propulsion, astrodynamics, extraterrestrial intelligence and Earth observations.