Assessing the quality of whole slide images in cytology from nuclei features

Abstract

Background and objective

Implementation of machine learning and artificial intelligence algorithms into digital pathology laboratories faces several challenges, notably the variation in whole slide image preparation protocols. The diversity of preparation pipelines forces algorithms to be protocol-dependant. Moreover, the error susceptibility of each stage in the preparation process implies a need of quality control tools. To address these challenges, this article introduces a straightforward, interpretable, and computationally efficient quality control module to ensure optimal algorithmic performance.

Methods

The proposed quality control module ensures algorithmic performance by representing an algorithm by a reference whole slide image preparation protocol validated on it. Then, inspired by data description methods, a preparation protocol is represented by nuclei feature distributions, obtained for several whole slide images it has produced. The quality of a preparation protocol is evaluated according to several reference preparation protocols, by comparing their feature distributions with a weighted distance.

Results

Through empirical analysis conducted on seven distinct preparation protocols, we demonstrated that the proposed method build a quality module that clearly discriminates each preparation. Additionally, we showed that this module performs well on more larger and realistic corpus from laboratories routine, detecting quality deviations.

Conclusion

Even if the proposed method necessitates minimal data and few computational resources, we showed that it is interpretable and relevant on realistic corpus from several laboratories' routine. We strongly believe in the necessity of quality control from the algorithmic perspective and hope this kind of approach will be extended to improve quality and reliability of digital pathology whole slide images.