Seed-specific expression of phosphatidate phosphohydrolases increases soybean oil content and seed weight

IF 6.1 1区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology for Biofuels Pub Date : 2025-02-24 DOI:10.1186/s13068-025-02620-x

Beibei Chen, Jianwu Li, Shuaibing Yao, Geliang Wang, Xuemin Wang

{"title":"Seed-specific expression of phosphatidate phosphohydrolases increases soybean oil content and seed weight","authors":"Beibei Chen, Jianwu Li, Shuaibing Yao, Geliang Wang, Xuemin Wang","doi":"10.1186/s13068-025-02620-x","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Soybean is a major oil crop and a primary protein source for livestock, and soybean oil is the most common input for biodiesel. Identifying genes that enhance soybean yield and oil content is crucial for breeding programs. Phosphatidic acid (PA) phosphohydrolase (PAH), which dephosphorylates PA to diacylglycerol (DAG), plays a critical role in lipid synthesis, and yet their potential in improving agronomic traits of oil crops remains unexplored.</p><h3>Results</h3><p>This study shows that seed-specific expression of <i>AtPAH1/2</i> enhances PA turnover into DAG and triacylglycerol (TAG) accumulation in soybean seeds. <i>PAH</i> overexpression upregulated the expression of DAG acyltransferase (<i>DGAT</i>) but suppressed phospholipid: DAG acyltransferase (<i>PDAT</i>). In addition, seed-specific expression of <i>AtPAH1/2</i> increases soybean seed size and weight. Furthermore, analysis of the variation of the soybean PAHs in 4414 soybean accessions indicated that the advantageous effects of <i>GmPAH</i>s on oil content and seed weight were selected during domestication.</p><h3>Conclusion</h3><p>These findings suggest that targeting <i>PAH</i>s represents a promising strategy for enhancing soybean seed oil content and yield in current cultivars and landraces soybeans.</p></div>","PeriodicalId":494,"journal":{"name":"Biotechnology for Biofuels","volume":"18 1","pages":""},"PeriodicalIF":6.1000,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://biotechnologyforbiofuels.biomedcentral.com/counter/pdf/10.1186/s13068-025-02620-x","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology for Biofuels","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1186/s13068-025-02620-x","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Soybean is a major oil crop and a primary protein source for livestock, and soybean oil is the most common input for biodiesel. Identifying genes that enhance soybean yield and oil content is crucial for breeding programs. Phosphatidic acid (PA) phosphohydrolase (PAH), which dephosphorylates PA to diacylglycerol (DAG), plays a critical role in lipid synthesis, and yet their potential in improving agronomic traits of oil crops remains unexplored.

Results

This study shows that seed-specific expression of AtPAH1/2 enhances PA turnover into DAG and triacylglycerol (TAG) accumulation in soybean seeds. PAH overexpression upregulated the expression of DAG acyltransferase (DGAT) but suppressed phospholipid: DAG acyltransferase (PDAT). In addition, seed-specific expression of AtPAH1/2 increases soybean seed size and weight. Furthermore, analysis of the variation of the soybean PAHs in 4414 soybean accessions indicated that the advantageous effects of GmPAHs on oil content and seed weight were selected during domestication.

Conclusion

These findings suggest that targeting PAHs represents a promising strategy for enhancing soybean seed oil content and yield in current cultivars and landraces soybeans.

查看原文本刊更多论文

磷脂酸磷酸化酶在种子中的特异性表达增加了豆油含量和种子重量

大豆是一种主要的油料作物和牲畜的主要蛋白质来源，大豆油是生物柴油最常见的投入物。确定提高大豆产量和含油量的基因对育种计划至关重要。磷脂酸（PA）磷酸水解酶（PAH）在油料作物的脂质合成中起着关键作用，但其在改善油料作物农艺性状方面的潜力尚未探明。结果本研究表明，AtPAH1/2的种子特异性表达可促进大豆种子中PA转化为DAG和三酰甘油（TAG）的积累。多环芳烃过表达上调DAG酰基转移酶（DGAT）表达，抑制磷脂：DAG酰基转移酶（PDAT）表达。此外，AtPAH1/2的种子特异性表达增加了大豆种子的大小和重量。此外，对4414份大豆材料中大豆多环芳烃的变异分析表明，GmPAHs在驯化过程中对大豆含油量和种子重产生了有利影响。结论以多环芳烃为靶点，是提高现有品种和地方品种大豆籽油含量和产量的有效途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biotechnology for Biofuels 工程技术-生物工程与应用微生物

自引率

0.00%

发文量

审稿时长

2.7 months

期刊介绍： Biotechnology for Biofuels is an open access peer-reviewed journal featuring high-quality studies describing technological and operational advances in the production of biofuels, chemicals and other bioproducts. The journal emphasizes understanding and advancing the application of biotechnology and synergistic operations to improve plants and biological conversion systems for the biological production of these products from biomass, intermediates derived from biomass, or CO2, as well as upstream or downstream operations that are integral to biological conversion of biomass. Biotechnology for Biofuels focuses on the following areas: • Development of terrestrial plant feedstocks • Development of algal feedstocks • Biomass pretreatment, fractionation and extraction for biological conversion • Enzyme engineering, production and analysis • Bacterial genetics, physiology and metabolic engineering • Fungal/yeast genetics, physiology and metabolic engineering • Fermentation, biocatalytic conversion and reaction dynamics • Biological production of chemicals and bioproducts from biomass • Anaerobic digestion, biohydrogen and bioelectricity • Bioprocess integration, techno-economic analysis, modelling and policy • Life cycle assessment and environmental impact analysis