Impact of biocontainment on small animal PET performance adapted for BSL-2/3 infectious disease imaging research.

IF 3.1 3区 医学 Q1 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Isabella Salerno, Nadia Benabdallah, Amanda Fears, Ryan Unnerstall, Lindsey Hauck, Sergey Komarov, Linda Cox, Hanwen Zhang, Kevin Poenicke, Joseph Aromando, Yuan-Chun Tai, Timothy Wencewicz, Deborah J Veis, Daniel L J Thorek
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引用次数: 0

Abstract

Background: Biocontainment protocols are critical for conducting infectious disease (ID) research, particularly when using small animal models in biosafety level (BSL) 2/3 environments. This study evaluates the impact of poly-methyl methacrylate (PMMA) containment vessels on the performance of preclinical positron emission tomography (PET) systems. We tested containment vessels designed with varying wall thicknesses (3, 6, and 9 mm) to simulate ID imaging facility equipment and protocols. Through the use of multicomponent phantoms and in vivo mouse models of Staphylococcus aureus infection, we assessed key performance metrics including count rate, image quality, activity recovery, and spatial resolution.

Results: The results indicate that the use of PMMA containment causes only minor reductions in imaging performance. The thickest PMMA (9 mm) led to a maximum 6.8% decrease in count rate, which remains well within the acceptable range of variation. Effects on spatial resolution were most noticeable for smaller structures within the phantom study, with a 19.65% difference in full width at half maximum (FWHM) for the thickest walled vessel. In vivo, using infected mice, the containment devices had modest effects on the task of activity concentration to be detected at the infection site, even with the thickest PMMA tube.

Conclusion: These findings suggest that PMMA biocontainment vessels have small but measurable impact on preclinical PET system performance, making them a viable and cost-effective solution for conducting infectious disease imaging under BSL-2/3 conditions. Specifically, the thinnest containment (3 mm) had only minor effects on all tested parameters, suggesting it is well-suited for use in ID enclosures while maintaining accurate qualitative and quantitative assessments. This approach may reduce the burden for fully separate and specialized modifications for BSL-3 imaging facilities, and can be broadly applied to preclinical research involving pathogenic organisms.

生物防护对适用于BSL-2/3传染病成像研究的小动物PET性能的影响
背景:生物控制方案对于开展传染病(ID)研究至关重要,特别是在生物安全水平(BSL) 2/3的环境中使用小动物模型时。本研究评估了聚甲基丙烯酸甲酯(PMMA)容器对临床前正电子发射断层扫描(PET)系统性能的影响。我们测试了设计为不同壁厚(3,6和9mm)的安全壳,以模拟ID成像设施设备和协议。通过使用多组分模型和金黄色葡萄球菌感染小鼠体内模型,我们评估了关键性能指标,包括计数率、图像质量、活动恢复和空间分辨率。结果:结果表明,使用PMMA容器只会导致成像性能的轻微降低。最厚的PMMA (9 mm)导致计数率最大下降6.8%,这仍然在可接受的变化范围内。在幻影研究中,对较小结构的空间分辨率的影响最为明显,最厚壁血管的半最大全宽度(FWHM)差异为19.65%。在体内,使用受感染的小鼠,即使使用最厚的PMMA管,密封装置对在感染部位检测活性浓度的任务也有适度的影响。结论:这些研究结果表明,PMMA生物容器对临床前PET系统性能的影响很小,但可测量,使其成为在BSL-2/3条件下进行传染病成像的可行且具有成本效益的解决方案。具体来说,最薄的容器(3毫米)对所有测试参数的影响很小,这表明它非常适合在ID外壳中使用,同时保持准确的定性和定量评估。该方法可减轻对BSL-3成像设备进行完全分离和专门修改的负担,可广泛应用于涉及病原生物的临床前研究。
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来源期刊
EJNMMI Research
EJNMMI Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING&nb-
CiteScore
5.90
自引率
3.10%
发文量
72
审稿时长
13 weeks
期刊介绍: EJNMMI Research publishes new basic, translational and clinical research in the field of nuclear medicine and molecular imaging. Regular features include original research articles, rapid communication of preliminary data on innovative research, interesting case reports, editorials, and letters to the editor. Educational articles on basic sciences, fundamental aspects and controversy related to pre-clinical and clinical research or ethical aspects of research are also welcome. Timely reviews provide updates on current applications, issues in imaging research and translational aspects of nuclear medicine and molecular imaging technologies. The main emphasis is placed on the development of targeted imaging with radiopharmaceuticals within the broader context of molecular probes to enhance understanding and characterisation of the complex biological processes underlying disease and to develop, test and guide new treatment modalities, including radionuclide therapy.
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